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当归内酯拮抗环孢菌素A 氢化可的松及抗肿瘤药物的免疫抑制作用 |
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冯景奇 柳钟勋
摘 要：目的：观察当归内酯（ASDL）对免疫抑制剂及抗肿瘤药物引起的免疫抑制作用的影响。方法：应用免疫抑制剂及抗肿瘤药物建立免疫功能低下的模型，使用不同剂量的ASDL进行体内外拮抗实验。结果：ASDL在62.5, 125.0, 250.0 μg/ml时，可部分或完全拮抗环孢菌素A、丝裂霉素C、5-氟尿嘧啶和阿糖胞苷对小鼠混合淋巴细胞培养反应(MLR)的轻度抑制作用(抑制50%以下)，部分拮抗氢化可的松对MLR的重度抑制(抑制50%以上)作用。ASDL 10及20 mg/(kg.d)×7，腹腔注射可拮抗环磷酰胺(CY, 80 mg/kg, s.c)对小鼠脾脏T淋巴细胞增殖、NK细胞活性、IL-2产生的免疫抑制作用。结论：当归内酯可通过不同途径拮抗化学因素引发的免疫抑制作用。
关键词：当归内酯 免疫抑制剂 抗肿瘤药物 混合淋巴细胞培养反应
分类号：R371-34
Antagonistic effect of Angelica Sinesis(Oliv.) Diels Lactone(ASDL) on the immunosuppressive response induced by Cyclosporin A, Hydrocortisone and antitumor agents
FENG Jing-Qi
Institute of Medical Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050
LIU Zhong-Xun
Institute of Medical Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050
Abstract：Objective: To study antagonistic effect of Angelica Sinesis(Oliv.) Diels Lactone (ASDL) on the immunosuppressive response induced by Cyclosporin A , Hydrocortisone and antitumor agents. Methods: Immunodeficient models were used in the experiments. Immunosuppressive response was induced by immunosuppressive and antitumor agents. In the different concentration of ASDL, antagonistic experiments were observed in vivo and vitro. Results: ASDL (62.5, 125.0, 250.0 μg/ml) could completely antagonize the mild inhibition (less than 50%) of murine mixed lymphocyte reaction (MLR) caused by Cyclosporin A, Mitomycin C, 5-Fluorouracil,Cytarabine and partly antagonize the serious inhibition (more than 50%) induced by Hydrcortisone in vitro .ASDL〔10 or 20 mg/(kg.d)×7, i.p〕 could antagoni inhibition of immunological function caused by Cyclophosphamide (CY,80 mg/kg, s.c), which could promote the declined T lymphocyte proliferation , restore the reduced NK cell cytotoxicity and production of IL-2.Conclusion: ASDL could antagonize immunosuppressive response induc[1] [2] 下一页
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