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雷公藤内酯醇诱导人淋巴细胞凋亡的作用与细胞周期的关系 |
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刘栋 杨俊伟 陈朝红 戴春笋 刘志红 黎磊石
摘 要 目的：通过观察雷公藤内酯醇诱导淋巴细胞发生凋亡与细胞周期的关系，探讨该化合物导致细胞凋亡作用的机制。方法：以人外周血T细胞为研究对象，选择流式细胞分析术、DNA凝胶电泳及DNA片段测定等作为检测细胞凋亡的方法。结果：雷公藤内酯醇只能造成活化的T细胞发生细胞凋亡，且与雷公藤内酯醇的浓度正相关；雷公藤内酯醇诱导活化T细胞发生细胞凋亡的作用与细胞活化的程度密切相关；细胞周期分析结果表明，雷公藤内酯醇只能造成S+G2/M期细胞发生细胞凋亡，却不能导致G0/G1期细胞发生细胞凋亡。结论：雷公藤内酯醇诱导淋巴细胞发生细胞凋亡的作用与其所处的细胞周期密切相关。
关键词 雷公藤内酯醇 细胞凋亡 细胞周期
中国图书分类号 R285.6
Relationship between cell cycle and apoptosis of activated T lymphocyctes
induced by triptolide
LIU Dong , YANG Jun-Wei, CHEN Zhao-Hong et al.
Research Institute of Nephrology,Jinling Hospital, School of Medicine,Nanjing University,Nanjing 210002
Abstract Objective:In order to explored the mechanisms of triptolide-induced apoptosis,the relationship between cell cycle and apoptosis of activated T cell induced were studied.Methods:Human peripheral T cells were stimulated with mitogen.To test the susceptibility to cell death by triptolide whether resulted from the process of coninuous cell cycling or ,alternatively,from the entry into a vulnerable stage of the cell cycle,T cell were cultured with agent that the cell at different stages.For the cell cycle analysis,cells were collected and stained with propidium iodide,then analyzed with a flow cytometer(EPICS XL,Coulter).In addition,apoptosis was confirmed with DNA fragmentation and membrane damage.Results:The degree of susceptibility to apoptosis correlated with the level of cell cycling as measured by thymidine incorporation during S phase;and DNA fragmentation associated with entry into S phase.The number of cells in S phase was proporational to amount of apoptotic cell induced by triptolide.Prevention of cell death correlated with delayed entry into S phase from G1 following triptolide added.Treatment of activative T cell with dBcAMP or forskolin,which block primary activated T cells in G1 phase ,also inhibited triptolide-induced cell death.In contrast,the DNA synthesis inhibitor aphidicolin ar[1] [2] 下一页
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