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牛膝多糖对人胸腔巨噬细胞的激活作用 |
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吕建新 俞 康 金丽琴 袁 谦 谢克俭 陆永绥
摘 要 目的：研究牛膝多糖对巨噬细胞的激活作用。方法：0.312、0.625、1.250、2.500和5.000 mg/ml牛膝多糖对体外培养的人胸腔巨噬细胞进行诱导培养24 h，用全自动生化分析仪测定胸腔巨噬细胞内乳酸脱氢酶与酸性磷酸酶活性，用放射免疫分析法测定胸腔巨噬细胞内肿瘤坏死因子-α与白细胞介素-6的含量。结果：牛膝多糖能上调胸腔巨噬细胞内乳酸脱氢酶和酸性磷酸酶活性，并能显著诱导胸腔巨噬细胞表达肿瘤坏死因子-α和白细胞介素-6，而且对肿瘤坏死因子-α的诱导表达具有显著的剂量效应。结论：牛膝多糖可诱导巨噬细胞表达细胞因子，并对巨噬细胞具有激活作用。
关键词 牛膝多糖 胸腔巨噬细胞 酶活性 细胞因子 激活作用
中国图书分类号 R392.12
The activated effect of achyrathes bidentata polysaccharides on thoracic cavity macrophages of human
LU Jian-Xin, YU Kang, JIN Li-Qin et al.
Department of Clinical Laboratory Medicine, Wenzhou Medical College, Wenzhou 325027
Abstract Objective:To study the activated effects of Achyrathes bidentata polysaccharides (ABPS) on thoracic cavity macrophages of human (hTMφ) .Methods：After the hTMφ were cultured with 0.312, 0.625, 1.250, 2.500, 5.000 mg/ml Achyrathes bidentata polysaccharides (ABPS)-RPMI1640 for 24 h in vitro. The activities of lactate dehydrogenase (LDH) and acid phosphatase (ACP) in the hTMφ were detected by automatic biochemistry analyzer, and the expression levels of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) in the hTMφ were analyzed by radioimmunoassay. Results：The activities of LDH and ACP increased in the hTMφ induced by ABPS, and the expression levels of TNF-α and IL-6 in the hTMφ induced by ABPS increased markedly too, and the expression of TNF-α had significantly dose effect. Conclusion：ABPS can induce the expression of cytokines in the macrophages, and activate the macrophages.
Key words Achyrathes bidentata polysaccharides Thoracic cavity macrophage Enzyme activity Cytokine Activation
牛膝多糖(Achyranthes bidentata polysaccharides,ABPS)是从牛膝(Achyranthes bidentata blume)根中分离提取得到的一种小分子水溶性的多糖化合物，其化学结构已经明确，糖基组成为果糖和葡萄糖，糖基摩尔比为8.7:1.0，平均分子量为1 400。已有研究报道ABPS对大鼠、小鼠均具有免疫调节作用，并提示ABPS可以启动和活化巨噬细胞(macrophages,Mφ)［1］。本文研究ABPS对体外[1] [2] 下一页
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