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脂多糖诱发急性肺炎时中性粒细胞碱性磷酸酶细胞化学定位观察 |
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姜晓丹 小林俊博 宋文光 濑口春道
摘 要：目的 探讨脂多糖诱发急性肺炎时，大白鼠中性粒细胞碱性磷酸酶(alkaline phosphatase,ALP)的超微定位及其在炎症过程中的相关功能。 方法 采用腹腔内(intraperitoneal,IP)注射及气管内(intratracheal,IT)滴注脂多糖(lipopolysaccharide,LPS,一种提自大肠杆菌的内毒素)的方式诱发大白鼠急性肺炎，使中性粒细胞在肺内不同部位集聚。用铈作为捕捉剂，以检测ALP活性。对照组为介质中除去底物或加入2mmol/L左旋咪唑。电镜下行酶活性定位观察。 结果 IP注射主要导致中性粒细胞在鼠肺肺泡隔毛细血管内集聚，而IT注射多致中性粒细胞聚集于肺泡腔中。在偶见的非炎区肺内中性粒细胞，ALP活性产物主要以小球状或管状的膜包颗粒存在于胞质中。LPS腹腔内注射组中，ALP活性除出现于胞质内，还呈现于中性粒细胞的质膜表面；在气管内给药组，与毛细血管内皮细胞或肺泡上皮细胞相接触的中性粒细胞质膜表面ALP活性有所增强，而肺泡腔的中性粒细胞质膜表面ALP活性减弱。在各组标本中，对照组均为ALP阴性。 结论 大鼠肺中性粒细胞ALP活性可经LPS注射而增强，其活性可能和细胞与细胞间的粘连、细胞外环境以及与细胞的功能状态有关。
关键词：超微细胞化学；中性粒细胞；碱性磷酸酶；脂多糖；大白鼠肺
分类号：Q538 文献标识码：A
文章编号：0529-1356(2000)01-52
STUDY ON CYTOCHEMICAL LOCALIZATION OF ALKALINE
PHOSPHATASE ACTIVITY IN NEUTROPHILS OF RAT
LUNG DURING THE LPS-INDUCED ACUTE PNEUMONIA
JIANG Xiao-dan
(Neurosurgery Laboratory,Zhujiang Hospital,The First Military Medical University)
Kobayashi T
(Department of Anatomy and Cell Biology,Kochi Medical School,783 Japan)
SONG Wen-guang
(Otorhinolaryngology Guangzhou 510282,China)
Seguchi H
(Department of Anatomy and Cell Biology,Kochi Medical School,783 Japan)
Abstract：Objective To explore the cytochemical localization of alkaline phosphatase(ALP) in neutrophils in the rat lung and the related function following the LPS-induced acute pneumonia. Methods The accumulations of the neutrophils in the different parts of the lung were induced by intraperitoneal(IP) or intratracheal(IT) injection of lipopolysaccharide(LPS,a kind of endotoxin from Escherichia coli).The detection of ALP activity was performed in a cerium-based reaction medium.The controls included a substrate-free medium or a medium containing 2mmol/L levamisole.The localization of the ALP activity was observed by a electron microscope. Results The IP-injection of LPS induced mainly a significant accumulation of the neutrophils within the capillaries of alveola[1] [2] [3] 下一页
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