|
嵌合性硫代磷酸修饰的反义c |
| |
王家宁 胡大一 马雅銮 高天祥 刘勃
摘 要 目的：探讨c-myc基因表达在血管平滑肌细胞增殖信号传导通路中的作用和采用反义c-myc寡脱氧核苷酸抑制血管平滑肌细胞(SMC)增殖的作用。方法：采用针对大鼠c-myc mRNA包括AUG在内的翻译起始区的前5个密码子的嵌合性硫代修饰的反义c-myc寡脱氧核苷酸(AS-ODN)，以lipofectin导入SMC后，通过细胞计数和3H-TdR掺入观察其对SMC增殖的抑制作用。结果：c-myc AS-ODN不仅对从静止状态刺激的SMC增殖有抑制作用，而且对处于增殖状态的SMC的增殖也有显著抑制作用，这种抑制作用可因加入正义c-myc ODN而减弱甚至消除，而且随着剂量的增加，抑制作用越来越明显。一次性应用c-myc AS-ODN可达到较持久的作用。而正义ODN和错配ODN对SMC增殖无影响。结论：c-myc AS-ODN以剂量依赖和序列特异方式抑制SMC增殖。c-myc基因表达在SMC增殖的信号传导通路中起重要作用。为采用c-myc AS-ODN抑制SMC增殖的反义战略防治再狭窄研究奠定了基础。
主题词 寡核苷酸类，反义；基因，MYC；肌，平滑；细胞
Inhibition of rat aortic smooth muscle cell proliferation by chimeric phosphorothioate
antisense c-myc oligodeoxynucleotide
WANG Jia-Ning, HU Da-Yi, MA Ya-Luan, GAO Tian-Xiang, LIU Bo
The Heart Cennter, Beijing Red Cross Chaoyang Hospital, Capital University of Medical Sciences，Beijing(100020)
Abstract AIM：Investigate the role of c-myc gene expression in the signal transduction pathways mediating smooth muscle cell (SMC) proliferation and the effect of chimeric phosphorothioate antisense c-myc oligodeoxynucleotide (AS-ODN) on rat aortic SMC proliferation.METHODS：Chimeric phosphorothioate c-myc AS-ODN targeting the 1st 5 codons of translation initiation region of c-myc mRNA was introduced by lipofectin and its inhibitory effect on SMC proliferation was observed by cell count and ［3H］-TdR incorporation. RESULTS：c-myc AS-ODN markedly suppressed proliferation of not only SMC stimulating from quiescent state but also exponential SMC. The inhibitory effect of c-myc AS-ODN on SMC proliferation was decreased and even abrogated by adding sense c-myc ODN. The larger dose there was, the greater inhibitory effect of c-myc AS-ODN on SMC proliferation there was. A single administration of c-myc AS－ODN had lasting action.Sense c－myc ODN and mismatch c-myc ODN had no significant effect on SMC proliferation compared with control. CONCLUSIONS：c-myc AS-ODN inhibited SMC proliferation in a dose-depend[1] [2] [3] 下一页
上一个医学论文： 非创伤缺血预处理对大鼠缺血再灌注心肌的作用
下一个医学论文： 碱性成纤维细胞生长因子对兔血管舒张功能及左旋精氨酸 一氧化氮途径的影响
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文