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沈阳地区不同人群HCV感染及HCV基因型分布研究 |
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黄 芬1 董亚珞2 王占英1 赵桂珍1 李 颖1 苏德元1
摘要 应用酶联免疫检测技术，检测沈阳地区3 902例不同人群血清的抗-HCV，并应用引物特异PCR法对其中100例血清标本进行HCV基因分型。结果：正常人群抗-HCV阳性率为0.42%～1.66%；输血后及散发性肝炎(除外HAV、HBV、EBV、CMV感染)、乙型肝炎、肝炎后肝硬化(除外HBV感染)、原发性肝癌等组抗-HCV阳性率显著高于无输血史的非肝病病人组(P<0.01)；100例血清标本中HCV-Ⅱ型感染占58%、HCV-Ⅲ型27%、Ⅱ/Ⅲ混合型14%、未分型1%，正常人群HCV感染者中HCV基因型以Ⅲ型为主(80%)，丙型肝炎后肝硬化病人HCV基因型以Ⅱ型为主(91.7%)。提示：各类肝病病人是HCV感染的高危人群；沈阳地区HCV感染以Ⅱ型为主，其次为Ⅲ型及Ⅱ/Ⅲ混合型，HCV基因型可能与丙型肝炎病情轻重有关。
关键词 HCV感染 HCV基因型
Study on HCV Infection and the Distribution of HCV Genotypes in Different Populations in Shenyang Area Huang Fen*,Dong Yaluo,Wang Zhanying,et al.* Department of Infectious Disease,The Second Affiliated Hospital,China Medical University,Shenyang 110003
Abstract 3 902 serum samples in different populations were tested for anti-HCV by ELISA.Among 100 samples of them,genotypes of HCV were detected by PCR with type-specific primers.The results showed:a)anti-HCV positive rates in general populations were 0.42%-1.66%;b)anti-HCV positive rates in the group of post-transfusion hepatitis and sporadic hepatitis (excluding HAV,HBV,EBV,CMV infection),hepatitis B post hepatitis cirrhosis (excluding HBV infection) and primary hepatocarcinoma were significantly higher than the group of patients without liver diseases or the group of patients without transfusion (P<0.01); c) in HCV genotyping,HCV-Ⅱ,HCV-Ⅲ,Ⅱ/Ⅲ mixed type were 58%,27%,14% respectively.The rate of infection with HCV-Ⅲ was 80% in normal population but 91.7% with type Ⅱ HCV in the group of patients with cirrhosis.These results suggested:(1)patients with different liver diseases were under high risk of HCV infection;(2)type HCV-Ⅱ was predominant followed by HCV-Ⅲ as well as Ⅱ/Ⅲ mixed in HCV genotype distribution of Shenyang area.Different genotypes of HCV might be related to the severity of hepatitis C.
Key words HCV infection HCV genotypes
丙型肝炎病毒(HCV)感染的分布呈全球性，自然人群中HCV感染率为0.7%～3.1%〔1〕，不同地区HCV感染率有较大差别。近年来发现HCV变异较大，存在多种基因型，HCV不同基因型与HCV感染的[1] [2] 下一页
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