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经椎间动脉栓塞法建立急性脊髓缺血损伤模型 |
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徐明 杨惠林 倪才方 唐天驷
摘 要：目的 应用经椎间动脉栓塞法，建立一种新型的急性脊髓缺血损伤(SCII)动物模型。方法 在数字减影技术（DSA）监视下，15只成年家犬行选择性脊髓动脉造影后，选用4种不同粒径的固体栓塞剂，经椎间动脉栓塞脊髓供血动脉，采用神经学、病理学和影像学方法评价。 结果 以小颗粒聚乙烯醇（PVA，直径118～154 μm）为栓塞剂所致脊髓缺血梗塞程度最严重，表现为双后肢肌力减至0～1级，二便失禁；栓塞段脊髓中、重度坏死；MRI示T1加权像脊髓增粗，T2 加权像出现明显高信号。 结论 经椎间动脉法可有效制备急性SCII动物模型，是一种在相关实验和临床研究中可供选择的较实用方法。
关键词：脊髓损伤，缺血性； 动脉； 栓塞； 模型
Establishment of animal model of acute spinal cord ischemic
injury via the intervertebral artery embolization
XU Ming，YANG Huilin， NI Caifang，et al.
（Dept. of Orthopaedic Surgery, The First Affiliated Hospital of Suzhou Medical College, Suzhou 215006, China）
Abstract：Objective To establish a new animal model of acute spinal cord ischemic injury(SCII) via the intervertebral artery embolization. Methods Fifteen adult mongrel dogs were divided into three groups mainly according to different sizes of the vascular embolizing agents. The changes in neurobehavioral outcome,histopathology and radiology were evaluated. Results The most severe spinal cord infarction occurred in the group using the polyvinyl alcohol ( PVA) particles, the diameter of which ranged from 118μm to 154μm. The irreversible impairment resulted in the marked motor function deficits in the hindlimbs and incontinence of stool and urine. Neuropathological abnormalities involved in moderate and severe necrosis. The characteristics of magnetic resonance imaging (MRI) were local cord enlargement on T1 weighted images and noticeable high signal intensity on T2 weighted images. Conclusions The establishment of SCII model in the dog via the intervertebral artery embolization is applicable and suitable for the SCII related research in basic medicine and clinical work.
Key words：Spinal cord injuries, ischemic； Arteries； Infarction； Model▲
脊髓缺血性损伤(SCII)并不少见。制作SCII动物模型的研究自Stenonis (1667)以来［1］，虽方式不断改进，但仍是当前国内外致力探讨的课题［2］。笔者在数字减影（DSA）设备监视下行选择性脊髓动脉造影和栓塞，建立急性SCII动物模型，这在国内外尚未见报道。
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