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高浓度胰岛素 葡萄糖对HepG2细胞蛋白激酶C活性的影响 |
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叶娟 李果 陈家伦
【摘要】 目的 研究高浓度胰岛素、葡萄糖对HepG2细胞蛋白激酶C(PKC)活性的影响。方法建立具胰岛素抵抗特性受体下调的HepG2(DR-HepG2)细胞模型，测定10-7mol/L胰岛素、25 mmol/L葡萄糖对未处理HepG2(NDR-HepG2)细和DR-HepG2细胞膜PKC、胞浆PKC活性的影响。结果DR-HepG2细胞未受高浓度胰岛素、葡萄糖刺激时膜和胞浆PKC活性均高于NDR-HepG2细胞(P＜0.05)。10-7mol/L胰岛素刺激NDR-HepG2细胞或DR-HepG2细胞，30秒和5～10分时膜PKC活性增高，胞浆PKC活性降低(P＜0.01)。25mmol/L葡萄糖引起二种不同状态下HepG2细胞膜PKC活性二相增高，胞浆PKC活性增高(NDR-HepG2细胞)或降低(DR-HepG2细胞)(P＜0.01)。10-7mol/L胰岛素和25 mmol/L葡萄糖同时刺激细胞，膜和胞浆PKC活性的变化与25mmol/L葡萄糖单独刺激时基本一致。结论 PKC上调与DR-HepG2细胞的胰岛素抵抗状态有关。10-7mol/L胰岛素或25 mmol/L葡萄糖刺激二种不同状态下的HepG2细胞，引起膜PKC活性二相增高，但对胞浆PKC活性的影响不完全相同；二者同时刺激主要通过葡萄糖介导的机制激活PKC。
【关键词】 蛋白激酶C 胰岛素 葡萄糖 HepG2细胞
Effects of high concentrations of insulin and glucose on the protein kinase C activities of HepG2 cells
YE Juan,LI Guo,CHEN Jialun
Shanghai Institute of Endocrinology,Shanghai,200025
【Abstract】 Objective To study the effec?s of high concentrations of insulin and glucose on the prot?in kinase C (PKC) activities of HepG2 cells. Methods A model of receptor down-regulated HepG2 (DR-HepG2) cells with characteristics of insulin resistance was developed. The membrane and cytosolic PKC activities of NDR-HepG2 cells and DR-HepG2 cells stimulated by 10-7mol/L insulin and 25mmol/L glucose were measured. Results Without stimulation by 10-7mol/L insulin or 25mmol/L glucose, the PKC activities of membrane and cytosol of the DR-HepG2 cells were higher than those observed in NDR-HepG2 cells (P＜0.05). 10-7mol/L insulin induced biphasic increases of membrane PKC activities by NDR-HepG2 cells and DR-HepG2 cells (P＜0.01), the initial increase being at 30 sec and the secondary at 5～10min. Meanwhile, the activities of cytosolic PKC reduced markedly. 25mmol/L glucose induced biphasic increases of the membrane PKC activities by NDR-HepG2 cells and DR-HepG2 cells (P＜0.01). At the same time the activities of cytosolic PKC increased slightly by NDR-HepG2 cells or decreased by DR-HepG2 cells. The effects of 25mmol[1] [2] [3] 下一页
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