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G CSF体外诱导新生小鼠心肌干细胞分化的研究 |
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rse transcription polymerase chain reaction （RTPCR）was performed to detect the expression of cardiac transcription factor GATA4 and cardiac structural gene βMHC.Results FCM and IFS showed that the primary cells partly expressed both Sca1 and CD 45.High purity cell fraction was successfully obtained by MACS，and FCM and IFS showed that its’ cell phenotype was Sca1+/CD 45－.RTPCR analysis indicated that the purified cells expressed cardiac transcription factor GATA4，but not cardiac structural gene βMHC.The purified cells were induced with GCSF and cultured for three weeks.During the process，neither single cell nor agglomerate cells synchronized contraction was observed.Three weeks after the induction FCM was again performed，and an express reduction of Sca1 in all five groups was observed.Cardiac structural gene βMHC still not detected in the induced cells by RTPCR.Conclusion High purity cell fraction which phenotype is Sca1+/ CD 45－ was successfully obtained by MACS system.An express reduction of Sca1 may associate with the prolongation of cell culture period.GCSF could not induce CSCs into cardiomyocytes.
Key words： Car上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] 下一页
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