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24

袁 琳 赵毓橘

摘要:外加24-表油菜素内酯(24-epiBL) 无论在光下或暗中均可促进盐藻细胞分裂数的增加,激动素只在光下具有这种作用。外界Ca2+浓度升高时,24-epiBL促进细胞分裂的效果更为明显,而EGTA可以抑制24-epiBL引起的促进作用。Verapamil、W7、环己酰亚胺均可抑制盐藻细胞分裂。Ca2+载体A23187在低浓度(0.25μmol/L)时具有促进分裂的作用。可以认为24-epiBL对低等单细胞藻类具有生理作用,并且,其促进盐藻细胞分裂的机制与激动素是不同的,它不仅与Ca2+有关,而且与钙调素也有较密切的关系。

关键词:24-表油菜素内酯,盐藻,Ca2+,钙调素
学科分类号:Q945

24-epibrassinolide Promoting the Cell Division of Dunaliella salina Relation to Ca2+ and Calmodulin

YUAN Lin and ZHAO Yu-Ju
(Shanghai Institute of Plant Physiology, Chinese Academy of Science,Shanghai 200032)

Abstract:24-epibrassinolide was found to promote the cell division of Dunaliella salina,at a concentration as low as 0.005mg/L, with an optimal concentration at about 0.5mg/L(Fig.1). In contrast to kinetin,which is effective only in light, 24-epiBL could promote cell division both in light and in the dark(Fig.3). With Ca2+, EGTA, A23187, and Verapamil we found that the promoting of cell division by 24-epiBL was related to Ca2+ and Ca2+ channel. The depression effect of W7 indicates that the cell division of Dunaliella salina relates to calmodulin (CaM) (Fig.9). These results may indicate that 24-epiBL could promote cell division of Dunaliella salina and has a different way from kinetin. Moreover, 24-epiBL has its effect on the cell division through increasing cytoplasm Ca2+ concentration.The results also imply that 24-epiBL regulated cell division through its effect on protein synthesis.

Key words: 24-epibrassinolide,Dunaliella salina,Ca2+,calmodulin (CaM)

  24-表油菜素内酯(24-epibrassinolide,24-epiBL)为油菜素甾醇类(brassinosteroids,BR)物质,这类物质在植物的生长发育过程中起着极为重要的作用,它们可以诱导绿豆上胚轴和黄瓜下胚轴的伸长,促进花粉管的生长,抑制根的生长,促进水稻谷粒的成熟等。自Grove等(1979)提取并鉴定了油菜素内酯的结构以来,人们相继在40种植物体中得到BR(Sakurai和Fujioka 1993,Sasse 1997),其中不仅有被子植物和裸子植物,也有低等植物的绿藻类,并且在植物的茎、叶、花和种子等器官中都发现了BR(Arteca 1995),可见它们在植物界中的分布是很广泛的。目前,已从自然界中分离和人工合成了60多种油菜素甾醇类化合物(Kim 1991 )。
  BR对植物细胞方面的作用结果尤其引人注目,它可以影响高等植物细胞的分

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