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急性肺损伤大鼠多核白细胞与肺组织 受体变化的相关性研究 |
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徐兴祥 孙耕耘 钱桂生 董解菊
［摘 要］ 目的：观察急性肺损伤(ALI)大鼠循环血多核白细胞(PMN)和肺组织β肾上腺素能受体(β-AR)的变化，并探讨二者β-AR变化的相关性。方法：静注内毒素复制大鼠ALI模型，循环血PMN及肺组织β-AR用放射性配基结合分析法测定。结果：静注内毒素后1、4、6 h，大鼠循环血PMN及肺组织β-AR的最大结合容量(Bmax)均明显低于对照组(P＜0.01)；静注内毒素后4 h, 6 h较1 h及6 h较4 h PMN β-AR的Bmax均显著降低(P＜0.05)，肺组织β-AR Bmax各内毒素组间差异不明显(P＞0.05)；循环血PMN和肺组织β-AR变化在程度上无相关性(P＞0.05)。结论：ALI时循环血PMN及肺组织β-AR数目均明显减少，其在ALI发生发展中可能起一定的作用，但ALI时外周血PMN β-AR不能作为观察肺组织β-AR变化的相关指标。
［主题词］ 内毒素类；肺；创伤和损伤；受体，肾上腺素能，β；中性白细胞；大鼠
［中图分类号］ R563 ［文献标识码］ A
［文章编号］1000-4718(2000)05-0432-04
Relativity of beta adrenoceptor changes between polymorphonuclear leukocytes and lung tissue in acute lung injury rats
XU Xing-xiang, SUN Geng-yun, QIAN Gui-sheng, DONG Jie-ju
(Department of Respiratory Disease, XinQiao Hospital, Chongqing 400037, China)
［Abstract］ AIM : To observe changes of beta-adrenoceptor (β-AR) and its relativity to polymorphonulear leukocytes (PMN) and acute lung injury (ALI) in rats. METHODS:ALI modle in rat was established by intravonous injection of E. Coli endotoxin (ET). β-AR was measured by radioligand binding assay with ［３H］-dihydroalprenlol.RESULTS:(1)Maxmal capacity of β-AR (Bmax) in both lung tissue and PMN decreased significantly at the lst, 4th and 6th hours after injection of ET. (2)No relativity was found between β-AR Bmax changes of lung tissue and β-AR Bmax changes of PMN. CONCLUSIONS:(1) Decrease of density of β-AR in lung tissue and PMN may played a role in the development of ALI. (2) The β-AR of PMN in circular blood can not be used as a relative index of β-AR changes of lung tissue in rats with ALI.
［MeSH］ Endotoxins; Lung; Wounds and injuries; Receptors, adrenergic, beta; Neutrophils; Rats
β肾上腺素能受体(beta adrenergic receptor, β-AR)作为细胞表面主要结合肾上腺素及异丙肾上腺素的受体，是信号转导系统的重要组成部分，它不仅在肺组织中介导许多重要的生理功能，而且参与细胞自身及整个机体的机能[1] [2] 下一页
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