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急性脑缺血大鼠边缘系统谷氨酸及其受体对下丘脑 |
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陈曼娥 何明利 蒋晓江
（400042 重庆，第三军医大学大坪医院野战外科研究所）
【摘要】 目的 观察脑缺血/再灌流过程中海马和下丘脑的谷氨酸、谷氨酸受体(GluR)的变化特征及其对下丘脑-垂体-肾上腺轴(HPA)活动的影响。方法 应用高效液相色谱、受体的放射配体结合分析、核酸原位杂交、放射免疫分析分别监测大脑中动脉闭塞大鼠海马和下丘脑的谷氨酸含量、促皮质释放激素(CRH)信使核糖核酸(mRNA)表达水平、血浆促肾上腺皮质激素(ACTH)浓度变化。结果 动脉闭塞15分钟，下丘脑、海马两部位的谷氨酸含量迅速增加，缺血1 小时达高峰，复流后迅速回落至基线水平；再灌流24小时再次中度升高，并持续至48小时后缓慢回降。缺血期海马、下丘脑的GluR下调，再灌流期呈现典型的上向同种特异性正向调节。缺血1小时颞皮质，海马、下丘脑等区CRHmRNA表达明显活跃，并持续至再灌流96小时，血浆ACTH浓度同步升高。再灌流损伤高峰期下丘脑谷氨酸含量与CRHmRNA阳性表达细胞数、血浆ACTH浓度间均呈显著正相关。结论 谷氨酸参与了急性脑缺血特定条件下HPA的异常活动，可能是一个重要的触发因子。
【关键词】 脑缺血 氨基酸 受体 下丘脑-垂体系统
Effects of glutamate, glutamate receptor in limbic system and hypothalamus on hypothalamus-pituitary-adrenal axis after rats MCAO Chen Mane He Mingli Jiang Xiaojiang, DaPing hospital,Research Institute of Field Surgery, Third Military Medical University, Chongqing 400042
【Abstract】 Objective To investigate the features of activity changes of glutamate(Glu), glutamate receptors(GluR) in hippocampus and hypothalamus during rats middle cerebral artery occlusion (MCAO) or reperfusion. Methods Using radio-legend binding assay of receptor (RBA),high-performance liquid chromatography (HPLC) and hybridization in situ and radioimmunometric assay, (RIA), we determined the dynamic changes of Glu content,GluR capacity and it′s Kd in cell membrane from the hippocampus of the ischemic brain and the hypothalamus CRHmRNA expression levels in every brain areas and ACTH concentrations in plasma at different time after middle cerebral artery occlusion(MCAO) or reperfusion. Results Glu content rapidly increased in the hippocampus and the hypothalamus at 15 minutes after MCAO, and reached the peak values at I1h, and rapidly decreased to the basic-line level after reperfusion. Glu content IR24h to IR48h, was elevated again moderately , and declined slowly 48 hours after reperfusion. The GluR was down-regulated and the affinity enhanced in during the ischemic period. During the reperfusion period, the [1] [2] 下一页
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