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肝缺血预处理保护作用与一氧化氮 内皮素 |
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吕平 陈道达 田源 张景辉 吴毅华
［摘 要］ 目的：研究一氧化氮/内皮素-1（NO/ET-1）失衡与肝缺血再灌注（I/R）损伤的关系以及肝缺血预处理（IPC）对NO/ET-1系统的调节作用。方法：采用鼠肝I/R模型，比较I/R组和IPC+I/R组NO/ET-1系统的变化情况及其与肝I/R损伤的关系。用RT-PCR检测再灌注2h内肝组织中是否有诱生性一氧化氮合酶（iNOS）mRNA表达。结果：再灌注急性期血浆NO代谢产物（NO-2/NO-3）降低、ET-1升高致NO/ET-1比值降低，血浆ALT、AST、LDH、TNF-α含量及肝组织丙二醛（MDA）含量增高，而肝组织ATP含量降低，肝损伤加重；肝IPC的保护作用与其升高NO-2/NO-3、降低ET-1，升高NO/ET-1比值有关；在上述肝组织中未测出有iNOS mRNA表达。结论：肝I/R损伤与NO/ET-1失衡有关，IPC对I/R急性期肝的保护作用可能是通过对NO/ET-1系统的调节作用而介导的，此时NO来源于原生性一氧化氮合酶（cNOS）而非iNOS。
［主题词］ 肝； 再灌注损伤； 局部缺血；一氧化氮；内皮缩血管肽类
［中图分类号］ R364.1； R333.4 ［文献标识码］ A
［文章编号］ 1000-4718(2000)10-0901-05
The protection of the hepatic ischemic preconditioning is concerned
with the NO/ET-1 system
LU Ping
（Department of General Surgery, Laboratory of General Surgery, Union Hospital, Tongji Medical Institute,）
CHEN Dao-da
（Department of General Surgery, Laboratory of General Surgery, Union Hospital, Tongji Medical Institute,）
TIAN Yuan
（Huazhong University of Science and Technology, Wuhan 430022, China）
ZHANG Jing-hui
（Huazhong University of Science and Technology, Wuhan 430022, China）
WU Yi-hua
（Huazhong University of Science and Technology, Wuhan 430022, China）
［Abstract］ AIM: To study the relationship between the disturbance of nitric oxide/endothelin-1(NO/ET-1) and hepatic ischemia/reperfusion(I/R) injury as well as the regulation of NO/ET-1 system by hepatic ischemic preconditioning(IPC). METHODS: The changes of NO/ET-1 system and their relationship with hepatic I/R injury were compared between I/R group and IPC+I/R group in a rat hepatic I/R model. Two hours after reperfusion, the liver tissues were detected by RT-PCR to see whether there was inducible nitric oxide synthase (iNOS) mRNA expression. RESULTS:In the acute phase of hepatic reperfusion, the ratio of NO/ET-1 was reduced, which was due to a significant reduction of NO-2/NO-3 (the metabolic product [1] [2] [3] 下一页
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