钒酸盐对实验性糖尿病大鼠肾脏的影响

艾智华　张忠辉

　　摘要　目的 观察钒酸盐(Va)对链脲霉素诱导的糖尿病(DM)大鼠肾脏功能和结构的影响。方法 正常大鼠和糖尿病大鼠自由饮用钒酸钠水12周后，测定肾功能，包括内生肌酐清除率(Ccr)、尿蛋白排泄量(UPE)，并取肾脏作光镜和电镜检查，测定肾小球平均面积(MGPA)和肾小球平均体积(MGV)。结果 DM大鼠12周时存在肾功能改变，Ccr和UPE增加，MGPA和MGV增大，并有肾脏超微结构受损，而钒酸盐治疗能明显改善DM大鼠的上述改变，且对正常大鼠肾功能无明显影响。结论 本研究提示Va治疗对DM大鼠肾病有防治作用，具有临床应用前景。
　　关键词　钒酸盐　糖尿病　肾功能

Effects of Vanadate on Renal Function in Diabetic Rats

Ai Zhihua,Zhang Zhonghui.Department of Endocrinology,
Southwest Hospitol,The Thind Military Medical University, Chongqing 400038

Abstract Objective: To study the effects of Vanadate(Va) on renal function,and structure in streptozotocin induced diabetic rats and to provide the experimental bases for clinical application of Va.Methods: Sodium orthovasnadate was administered to normal and diabetic rats.24h urinary protein excretion(UPE) and clearance of creatinine(Ccr) were determined in normal rats and diabetic rats at 12nd week,and the kidaneys were processed for morphological examination with light and eletron microscopes.Mean glomerular plane area (MGPA) and mean glomerular volume(MGV) were calculated.Results: Ccr、LKW、MGPA and MGV increased at 12nd week in diabetic rats.The electron microscopic results showed that the ultrastructures of kidney were significanty deranged in diabetic rats.Ccr、LKW、MGP and MGV in Va-treated diabetic rats decreased to approach normal level,and the pathologic changes of kidney were improved significantly.In Va-treated noraml rats,no changes were found in renal function.No increase of plasma insulin was found.Conclusion: Va could contribute to the improvement of diabetic nephropathy in diabetic rats.
Key words Vanadatd Diabetes Renal function

　　钒酸盐(Va)具有广泛的模拟胰岛素作用［1］，能稳定有效地控制血糖、缓解胰岛素抵抗，而不增加血浆胰岛素水平［2，3］，成为有希望的糖尿病治疗药物。目前关于Va对肾脏功能影响的研究不多。本试验旨在为Va的临床应用提供实验依据，而观察了Va对正常大鼠及糖尿病大鼠肾脏的影响。
材料和方法
1　实验分组
　　采用雄性Wister大鼠，随机分成4组。
　　NC组：正常对照组，自由饮用NaCl水(80mmol/L)。
　　CV组：正常饮钒组，自由饮用含0.7mg/ml正钒酸钠(Sodium orthovasnadate,

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