系统性红斑狼疮病人血T B淋巴细胞Bcl

刘亚光　赵湘茹　尹培达　石成钢　孔庆瑜

　　中国图书分类号　R593.241
　　摘　要　目的：探讨Bcl-2在系统性红斑狼疮(SLE)发病机制中作用。方法：采用流式细胞仪双标记法检测31例SLE病人外周血T、B细胞Bcl-2蛋白表达。结果：发现活动期SLE病人CD3+、CD4+和CD8+T细胞Bcl-2蛋白表达明显高于非活动期SLE病人、其他疾病组和正常对照组。CD19+B细胞Bcl-2蛋白表达在各组之间并无统计学差异。CD3+T细胞Bcl-2蛋白表达的平均荧光强度(MFI)与SLE疾病活动指数(SLEDAI)成正相关关系(r=0.819 5，P＜0.01)，而与血沉、补体C3、C4水平及自身抗体(ANA、抗ds-DNA、抗Sm)无相关。结论：提示T细胞Bcl-2异常表达在SLE疾病活动的过程中起着重要作用。
　　关键词　系统性红斑狼疮　细胞凋亡　Bcl-2

Expression of Bcl-2 of peripheral blood T，B lymphocytes in patients with systemic lupus erythematosus

LIU Ya-Guang,ZHAO Xiang-Ru，YIN Pei-Da et al.
Division of Rheumatology,First Affiliated Hospital,Sun Yat-Sen Univerisity of Medical Sciences,Guangzhou 510080

　　Abstract　Objective:In order to investigate Bcl-2 expression in the pathogenesis of Systemic lupus erythematosus(SLE).Methods:Tested Bcl-2 protein levels in T,B cells of 31 patients with SLE by two-colourcytofluorography.Results:Compared with inactive SLE patients,normal controls and other non-SLE patients,a proposition of T cells (including CD3+,CD4+ and CD8+ subgroups) expressed Bcl-2 protein increased significantly in active SLE patients.However,Bcl-2 protein levels did not statistically differentiate in CD19+ B cells among all groups.Mean fluorescence intensity(MFI) of Bcl-2 protein on CD3+ T cells,which wasn t related to serological indices,was positively related to the SLE disease activity index(SLEDA) among SLE patients.Conclusion:Abnormal expression of Bcl-2 in T cells might play an important role in the active stage of SLE patients.
　　Key words　Systemic lupus erythematosus(SLE)　Apoptosis　Bcl-2

　　Bcl-2基因(B cell lymphoma/leukemia-2 gene)是人们在研究B细胞淋巴瘤中发现的一种异常表达的原癌基因，位于第18号染色体短臂21.3(18 q21.3)［1］。近年发现，Bcl-2为一种细胞凋亡抑制基因。Bcl-2转基因鼠自身反应性B细胞克隆消除功能抑制，B细胞记忆和生存期均延长，可产生许多自身抗体，形成SLE样病损，因而有人称之为自身免疫基因［2］。我们检测了SLE病人外周血淋巴细胞Bcl-2表达水平，探讨其临床意义。

1　病例与方法
1.1　对象　31例病例均符合1982年美国风湿病协会SLE分类修订标准。其中女性26例，平均年龄31±8岁(18～58岁)。几乎所有病人在受检时均用过强的松10～30 mg/d。参照唐氏标准

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