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热休克蛋白70与家兔颈总动脉球囊损伤后增殖细胞核抗原表达的关系 |
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郭远林 王瑞英 任江华 曹茂银 宋长杰
摘要 目的：研究热休克蛋白70(HSP70)与经皮冠状动脉腔内成形术后再狭窄形成中细胞增生的关系。方法：复制家兔颈总动脉球囊损伤模型，用免疫组化方法检测术后3、9、14、28 d损伤动脉壁中HSP70和增殖细胞核抗原(PCNA)表达水平；用计算机图像分析系统定量，并对两者进行相关分析。结果：损伤动脉壁HSP70及PCNA检测呈阳性，正常动脉呈阴性。9 d时两者表达指数均较各自3 d时显著升高，14 d均达峰值，28 d显著降至3 d时水平。时相相关分析两者显著正相关。结论：细胞增生活性升高伴随HSP70的产生增多；动脉损伤时机体可能通过HSP70合成增多而促进细胞再生，一定程度上促成再狭窄。
主题词 热休克蛋白质类；抗原；动脉
The relationship between expression of HSP70 and
PCNA in ballooned rabbit carotid
GUO Yuan-Lin, WANG Rui-Ying, REN Jiang-Hua，CAO Mao-Yin, SONG Chang-Jie
Department of Cardiology, The Second Affiliated Hospital, Hubei Medical University, Wuhan (430071)
Abstract AIM:To investigate the expression of heat shock protein 70 (HSP70) involving in the development of restenosis after percutaneous transluminal coronary angioplasty (PTCA).METHOD：The rabbit carotid balloon injury model was used.Six animals were killed at 3 days, six at 9 days, six at 14 days and six at 28 days after injury respectively, and the ballooned carotid segments were removed.Then, immunohistochemistry combined with computer image analysis was used to determine HSP70-expression index (HEI) and proliferating cell nuclear antigen (PCNA)-expression index (PEI) in injured arteries.RESULTS：Both stainnings were negative in normal vessels while positive in injured vessels.HEI and PEI significantly increased at day 9 compared with day 3 and reached their maxiums at day 14, then declined at day 28 significantly.The time course of cell proliferation was significantly positive correlated with that of the HSP70-expression.CONCLUSION：Cell proliferation is accompanied by production of HSP70.It seems that body may induce cell proliferation by increasing the synthesis of HSP70, thus to accelerate the repairment of injured artery.
MeSH Heat-shock proteins; Antigens; Arteries
经皮冠状动脉腔内成形术(percutaneous transluminal coronary angioplasty, PTCA)后再狭窄率高、顽固、难治，典型病理学特征是中膜平滑肌细胞向内膜迁移、增生。研究表明[1] [2] 下一页
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