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氧反常心肌细胞内游离钙浓度的变化及丹参素和SOD的影响 |
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朱甫祥 刘国美 杨志宏 王孝铭 马丽英
摘要 目的：观察心肌细胞氧反常时细胞内的游离钙浓度的变化，探讨丹参素和SOD对细胞内的游离钙浓度的影响。方法： 体外分离成年大鼠心肌细胞复制氧反常模型，缺氧40 min后给氧20 min，用钙荧光探针（Fluo-3）分别测定了正常大鼠心肌细胞(Control,n=11)、缺氧40 min(H40 min,n=11)以及缺氧40 min再给氧20 min(H40 min R 20min,n=12)的大鼠心肌细胞内的游离钙浓度，并观察了丹参素(H40minR20min+DS,n=10)和SOD(H40minR20min+SOD,n=11)对氧反常的心肌细胞内的游离钙浓度的影响，并用显微镜观察了细胞杆形率的改变。结果：氧反常组的细胞内的游离钙浓度（596.71±20.29 nmol/L）明显比正常对照和单纯缺氧40 min的高（158.67±5.01和328.51±13.86 nmol/L，P＜0.01）。加入丹参素和SOD，可明显降低氧反常细胞内的游离钙的浓度（分别为254.29±8.83和300.04±13.04 nmol/L，P＜0.01）。细胞杆形率亦有类似的改变。结论：氧反常时心肌细胞发生明显的钙超载，丹参素和SOD对细胞内钙超载具有一定的防治作用。
主题词 心肌；氧；钙；丹参；超氧化物歧化酶
Effects of Salvia Miltiorrhizae and SOD on Ca2+-overloaded
rat myocardial cells in oxygen paradox
ZHU Fu-Xiang,LIU Guo-Mei,YANG Zhi-Hong,WANG Xiao-Ming,MA Li-Ying
Institute of Radiation Medicine,Beijing(100850)
Abstract AIM:To observe the changes of intracellular free calcium concentration in oxygen-paradoxed rat myocardial cells and to discuss the protective effects of Salvia Miltiorrhizae (DS) and SOD on Ca2+-overloaded rat myocardial cells.METHODS: Adult rat myocardial cells was isolated in vitro and oxygen paradox model was made.Selecting 40 min as hypoxia period followed by 20 min of reoxygenation,a new calcium fluoresence probe (Fluo-3) was used to detect the intracellular free calcium concentration in control (n=11)、H40min (hypoxia,n =11) and H40minR20min (hypoxia+reperfusion,n=12) groups respectively.We also looked into the rod-shaped rates of myocardial cells with microscope.In addition,the effect of Salvia Miltiorrhizae (n=10) and SOD (n=11) on intracellular free calcium concentration and rod-shaped rate of oxygen-paradoxed myocardial cells were observed.RESULTS: The intracellular free calcium concentration of H40minR20min group increased far beyond the control and H40min groups (596.71±20.29 nmol/L vs 158.67±5.01 and 328.51±13.86 nmol/L,P＜0.01),[1] [2] [3] 下一页
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