【摘要】 目的:观察胰岛素样生长因子I(IGFI)、IGFI受体(IGFIR)在人退行性变腰椎间盘组织中的表达，探讨其生物学意义。方法：免疫组化(SP)法分别检测IGFI、IGFIR、增殖细胞核抗原(PCNA)在正常对照组(A组，n=16)、退行性变椎间盘组(B组，n=53)中的表达;TUNEL法检测两组椎间盘细胞凋亡情况。结果：IGFI、IGFIR在B组中表达低于A组，两组之间差异显著(P<0.05);细胞增殖指数(PI)B组低于A组，两组之间差异显著(P<0.05);细胞凋亡指数(AI)B组高于A组，两组之间差异显著(P<0.05);IGFI、IGFIR的表达与PI呈正相关(rs=0.59,rs=0.56,均P<0.01)、与AI呈负相关(rs=-0.60,rs=-0.57,均P<0.01);PI、AI值在IGFI、IGFIR阴性和阳性组有显著差异(均P<0.05)。结论：IGFI、IGFIR的异常表达与人腰椎间盘退行性变密切相关;IGFI、IGFIR促进细胞增殖，抑制细胞凋亡，在人腰椎间盘退行性变过程中起重要作用，IGFI、IGFIR异常表达可能导致椎间盘退行性变的发生。
【关键词】 胰岛素样生长因子I; 胰岛素样生长因子I受体; 椎间盘退行性变
Expression and biological significance of IGFI and IGFIR
in human degenerative intervertebral discs
XIE Yang1, WU Xiaotao2(1. School of Clinical Medicine, Southeast University, Nanjing 210009, China; 2. Department of Orthopaedics, Zhongda Hospital, Southeast University, Nanjing 210009, China)
[Abstract] Objective: To observe the expression of IGFI and IGFIR in human degenerative intervertebral discs and to investigate its biological significance. Methods: 69 patients were divided into two groups: 16 cases of normal intervertebral discs in group A and 53 cases of degenerative intervertebral discs in group B. The expression of IGFI，IGFR, PCNA and apoptosis were detected by immunohistochemistry and TUNEL. Results: The expression rates of IGFI and IGFIR in group B was lower than those in group A, the difference was significant between them(P<0.05). Proliferative index(PI) in group B was lower than that in group A, the difference was significant between them(P<0.05). Apoptotic index(AI) in group B was significant higher than that in group A(P<0.05). The expression of IGFI，IGFIR had positive correlation with PI(rs=0.59, rs=0.56, all P<0.01) and negative correlation with AI(rs=0.60, rs=0.57, all P<0.01). There was significant difference in PI and AI between IGFI, IGFIR positive expression group and negative expressi
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