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糜若然 王慧 瞿全新 郑德先 岳天孚 赵钧铭
摘 要：目的 探讨bcl-2反义脱氧寡核苷酸（ODN）及反义RNA对人宫颈癌体外治疗的效果。方法 应用反义技术进行人宫颈癌Hela细胞生长抑制及凋亡诱导。结果 Hela细胞中bcl-2 mRNA及蛋白均呈高表达。bcl-2反义ODN可有效抑制Hela细胞生长及降低其内源性bcl-2蛋白的表达，同时诱导其凋亡；bcl-2反义ODN 浓度为10 μmol/L时抑制效果最明显，抑制作用在转染后24 h效果最明显，持续至96 h后逐渐减弱，细胞凋亡程度与ODN用量呈区域性正相关；重组逆转录病毒载体（pDOR-AB）能有效将bcl-2反义RNA导入Hela细胞实现稳定整合，同时降低bcl-2 mRNA及蛋白表达，抑制Hela细胞生长并诱导其凋亡。结论 应用反义技术于体外治疗宫颈癌有一定效果，bcl-2基因作为优选靶向基因具有可行性，为进一步于体内治疗研究提供了理论基础和实验依据。
关键词：宫颈肿瘤； 寡核苷酸类，反义； 原致癌基因蛋白质类； 转染； 基因，bcl-2
The Inhibitory Effect of bcl-2 Antisense Oligodeoxynucleotide/RNA on the Growth of Cervical Carcinoma in vitro
MI Ruoran WANG Hui， QU Quanxin et al
（Department of Obstetrics and Gynecology, General Hospital, Tianjin Medical University, Tianjin 300052, China）
Abstract：Objective To study the therapeutic effect of bcl-2 antisense oligodeoxynucleotide（ODN）/RNA human cervical carcinoma in vitro. Methods To transfect bcl-2 antisense ODN/RNA into Hela cells with antisense technology and observe the growth and apoptotic modulation of Hela cells. Results The expression of bcl-2 protein and mRNA was higher in Hela cells； the bcl-2 specific antisense ODN significantly decreased bcl-2 protein expression and inhibit the growth of Hela cells. The strongest inhibitory effect was at 24 hours after transfection, the optimal concentration of antisense ODN was 10 μmol/L. The results indicated that the inhibitory effect of bcl-2 antisense ODN on cell growth and survival rate is dose dependent； the recombinant retroviral vector pDOR-AB can transfer antisense bcl-2 gene into the genome of Hela cells, bcl-2 protein and mRNA expressions were significantly decreased after transfected，it demonstrated bcl-2 antisense RNA can significantly inhibit the growth of Hela cells and induce their apoptosis. Conclusion The results demonstrate the feasibility of bcl-2 gene as a target for antisense gene therapy of human cervical carcinoma and provide the theoretical fo[1] [2] 下一页
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