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离体异位子宫内膜细胞中单核细胞趋化蛋白 |
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高颖 罗丽兰 何福仙
【摘要】 目的 探讨异位子宫内膜细胞产生单核细胞趋化蛋白-1(MCP-1)的临床意义。方法 收集15例子宫内膜异位症患者的异位内膜组织进行体外细胞培养，分别加入纯培养液（对照组）、含白细胞介素-1β（IL-1β）2 ng/ml（IL-1β诱导组）及肿瘤坏死因子α（TNF-α）20 mg/ml（TNF-α诱导组）的培养液培养4小时，应用斑点杂交、免疫组织化学及夹心酶联免疫吸附试验检测各组异位细胞中MCP-1 mRNA蛋白的表达水平及其培养上清中MCP-1的含量。结果 与对照组相比，异位内膜细胞经IL-1β及TNF-α作用后表达的MCP-1 mRNA和蛋白水平显著增加(P<0.01，P<0.05)；培养上清中MCP-1的含量也显著增加(P<0.01，P<0.05)。结论 IL-1β及TNF-α可刺激异位内膜细胞中MCP-1的表达显著增加，这可能和子宫内膜异位症的病变发展有关。
【关键词】 子宫内膜异位症 细胞，培养的 单核细胞化学吸引蛋白质 白细胞介素1肿瘤坏死因子
Determination of Monocyte Chemotactic Protein-1
in Cultured Endometriotic Cells
GAO Ying*, LUO Lilan, HE Fuxian.
* Union Hospital, Tongji Medical University, Wuhan 430022
【Abstract】 Objective To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues. Methods Expressions of MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis, immunohistochemical method ［streptavidin biotin-peroxidase complex (SABC)］ and enzyme linked immunosorbent assay (ELISA) methods in cultured endometriotic cells with median (controls) or with interleukin-1β(IL-1β)2 ng/ml (IL-1β group), and tumor necrosis factor-α(TNF-α)20 mg/ml(TNF-α group) respectively. The endometriotic tissues were sourced from 15 patients with endometriosis. Meanwhile, the MCP-1 protein content of cultured supernatent was also measured. Results After exposured to IL-1β or TNF-α, the expression of MCP-1 mRNA and MEP-1 protein in the endometriotic cells were significantly higher than that in the control group (P<0.01, P<0.05 respectively); so was the MCP-1 protien content of supernatent. Conclusions IL-1 β and TNF-α can up-regulate the expression of MCP-1 in endometriotic cells, which may be related to the development of emdometriosis.
【Key words】 Endometriosis Cell, cultured Monocyte[1] [2] 下一页
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