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同时测定血清中苯巴比妥 苯妥英及卡马西平的反相高效液相色谱法 |
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杜国有 吴 闯
摘要 为了探讨高效液相色谱法同时测定苯巴比妥(PB)、苯妥英(PHT)、卡马西平(CBZ)血药浓度的最佳实验条件，将标本在pH7.6条件下用二氯甲烷提取后经高纯度氮气在50℃水浴中吹干，再用甲醇重溶后进行色谱分析。色谱条件：YWG-C18柱(250mm×4.6mm，10μm)，流动相甲醇：水为60∶40，流速1ml/min，柱温40℃，波长254nm。结果PB、PHT、CBZ的最低检出浓度各为0.67μg/ml、0.94μg/ml和0.31μg/ml。在三个不同水平下，PB、PHT、CBZ的平均回收率各为98.14%、96.72%和99.36%，日内和日间变异均低于6.25%，内源性物质及10种常用药物未见干扰，在分析范围内具有良好的线性关系。该方法操作简便、快捷、准确，可满足临床和科研的需要。
关键词 苯巴比妥；苯妥英；卡马西平；血液；高效液相色谱法；药物监测
Simultaneous determination of phenobarbital(PB),
phenytoin(PHT) and carbamazepine(CBZ) in serum by
reversed-phase high-performance liquid chromatography(RP-HPLC)
Du Guoyou
Worker s Hospital of Zhongshan City, Guangdong Province (Zhongshan 528400)
To discuss the optimum experimental conditions of a simultaneous RP-HPLC determination of the concentration of PB, PHT and CBZ, these three drugs were extracted from serum at pH 7.6 with dichloromethane,blown dry at 50℃ under pure nitrogen gas, and reconstituted with methanol and injected in a YWG-C18(250mm×4.6mm, 10μm), column for analysis at wave length of 254 mm. The mobile phase used was methanol 60: water 40(V/V), with a flow rate of 1ml/min. The column temperature was 40℃. Results showed that the minimal detectable concentration for PB, PHT and CBZ were 0.67μg/ml, 0.94μg/ml and 0.31μg/ml, respectively. The average recovery rate at three different levels for PB, PHT and CBZ were 98.14%, 96.72% and 99.365, respectively. The within-day and day-to-day RSD were below 6.25%. Endogenous compounds and ten routine drugs were not found to interfere with this chromatographic procedure. The analytical range was linear from 5-80μg/ml for PB, from 5-50μg/ml for PHT and from 2-32μg/ml for CBZ. It is suggested that this manipulation is quick, simple, and accurate, and therefore suitable for clinics and scientific research.
Key words phenobarbital; phenytoin; carbamazepine; Blood; high-performance liquid ch[1] [2] 下一页
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