【摘要】 目的 观察含何首乌血清对人胚肺二倍体成纤维细胞(2BS)超氧化物歧化酶(SOD)活性、c-myc mRNA表达及端粒活性的影响。方法 采用含何首乌血清对2BS细胞株进行处理，用黄嘌呤氧化酶法检测SOD活性，RT-PCR检测c-myc mRNA表达，TRAP-ELISA检测端粒酶活性。结果 与正常对照组相比，用药组2BS细胞SOD活性明显增加(P<0.05)，c-myc mRNA表达无变化，端粒酶活性显著增加(P均<0.05)。结论 含何首乌血清对2BS细胞具有抗衰老作用，其机理可能与促进自由基的清除和激活端粒酶活性有关。
【关键词】 首乌;血清;药理学;人胚肺二倍成纤维细胞;超氧化物歧化酶;c-myc;端粒，末端转移酶
Effects of Tuber Fleeceflower Root serum on telomerase activity of human fetal lung diploid fibroblasts
LI Chao-gan, WEI Xing, NONG Song, WEI Yao-dong, ZHANG Shu-qiu
(Department of Biochemistry and Molecular Biology, Youjiang Medical College for Nationalities, Baise, Guangxi 533000, China)
Abstract: Objective To observe effects of Tuber Fleeceflower Root serum on activity of SOD, the expression of c-myc mRNA and the activity of telomerase of human fetal lung diploid fibroblasts (2BS). Methods 2BS cell lines were treated with Tuber Fleeceflower Root serum. The activity of c-myc mRNA was detected by semi-quantitive RT-PCR. A TRAP-ELISA method was used to measure the telomerase activity. Results Compared with those in the control group, the activity of SOD increased obviously (P<0.05), the expression of c-myc mRNA remained on the same level, and the telomerae activity were enhanced significantly(P<0.05) in the drug group. Conclusion Tuber Fleeceflower Root serum can postpone senescence of 2BS, its mechanism may be associated with enhancement elimination of free radicals and activation of telomerase.
Key words: Tuber Fleeceflower Root; srum; pharmacology; human fetal lung diploid fibroblasts; superoxide dismutase; c-myc; telomerase
何首乌又称首乌、赤首乌、乌肝石等，为蓼科植物何首乌(Polygonum multiflorum Thunb.)的块根，具有补肝肾、益筋血、壮筋骨、乌须发之功效。研究表明[1]，何首乌具有抗衰老、保护神经、增强免疫功能、降低血脂、抗动脉粥样硬化、抗心肌缺血、保肝、抗癌、抗菌等作用。在正常体细胞中，由于端粒的缩短及缺乏端粒酶的表达，细胞逐渐衰老。仅有极少数的细胞因为端粒酶在某种因子的作用下偶然激活，持续合成端粒DNA序列使细胞绕过M2退化期，获得无限增殖能力，从而逃避了程序性老化。端粒酶活性的调节可为抗衰老的研究提供一条新途径[2]。本组研究何首乌血清药物对人胚肺二倍成纤维细胞(2BS)内超氧化物歧化酶(SOD)活性、c-myc表达及端粒酶活性的影响，以探讨其抗衰老的可能机理，为抗衰
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