脑室内注射胰岛素对大鼠全脑缺血后海马CA1区Bcl

　　摘要：目的 观察胰岛素对全脑缺血后海马CA1区Bcl2 mRNA含量变化及其蛋白表达的影响，探讨胰岛素保护作用的机制。方法 利用4VO法制作大鼠全脑缺血模型。造成脑缺血15min后行再灌注 ，治疗组于再灌注后即刻经脑室注入1u胰岛素(10μL，1×105u/L)，缺血组则经脑室注入10μL生理盐水。利用反转录PCR(RTPCR)、免疫组化及Westernblot法分别于全脑缺血后1、3、5d观察海马CA1区Bcl2蛋白表达及其mRNA相对含量的变化。结果 全脑缺血后1、3、5d治疗组海马CA1区可见呈阳性表达的Bcl2蛋白，而缺血组海马CA1区Bcl2蛋白的表达则呈阴性;Westernblot分析，全脑缺血后1、3、5d治疗组海马CA1区Bcl2蛋白电泳条带密度分别为2890.791±213.616，3147.396±243.561和2594.834±204.736，明显高于缺血组的743.376±84.123，804.637±64.547和637.867±54.394(P<0.01)。全脑缺血后1、3、5d缺血组及胰岛素治疗组海马CA1区Bcl2 mRNA相对含量则未见明显差异。结论 全脑缺血后脑室内注入胰岛素可能通过促进海马CA1区神经元Bcl2基因的翻译途径从而促进Bcl2蛋白表达，这可能是其对全脑缺血后海马CA1区神经元产生中枢直接保护作用的主要机制之一 。

　　关键词：胰岛素;脑缺血;Bcl2

　　The effect of intraventricular administration of insulin on expression of Bcl2 mRNA and protein in rat hippocampal CA1 region following global brain ischemia

　　Li Bing, Zhang Xiang, Jiang Xiaofan, Wang Yangang, Zhang Ge

　　(Department of Neurosurgery, Xijing Hospital of Fourth Military Medical University, Xian 710032, China)

　　ABSTRACT: Objective To investigate the mechanism of neuroprotective effect of insulin on hippocampal CA1 region, the relatively content of Bcl2 mRNA and the expression of Bcl2 protein in rat hippocampal CA1 region following global brain ischemia were observed. Methods Fourvessel occlusion was used to establish the rat global brain ischemic model. After 15 minutes brain ischemia, 1 unit insulin (10μL, 1×105u/L) was injected into the cerebral ventricular immediately post reperfusion in treated group, while, 10μL NaCl (90g/L) solution was injected into the cerebral ventricular at the same time in ischemic group. The relatively content of Bcl2 mRNA and the expression of Bcl2 protein in hippocampal CA1 region had been detected by reverse transcription polymerase chain reaction (RTPCR), immunocytochemistry, and Westernblot, respectively. Results The expression of Bcl2 protein was negative in ischemic group and was positive in treated group, in 1, 3, 5 day post brain ischemia. While, Weste

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