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LPS对人胚胎胰岛分泌IL |
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孙 田志刚 杨贵贞 张建华
摘 要 目的：为了探讨免疫介质LPS对胚胎胰岛分泌IL-6以及对胰岛功能的影响，以便为研究免疫系统与内分泌系统的内在联系提供依据。方法：采用常规消化方法分离人胚胎胰岛并对其进行原代培养，加入浓度为10 mg/L LPS，经不同时相收获上清，测定IL-6、胰岛素、胰高血糖素含量和IL-6 McAb中和试验，对LPS刺激和对照组胰岛RNA进行IL-6 RNA 斑点杂交。结果：人胚胎胰岛经LPS刺激分泌IL-6的能力明显加强，在体外培养8 d，IL-6含量达高峰，为对照组的145倍；人胚胎胰岛在换新鲜培养液后用LPS二次刺激，仍可继续分泌高效价的IL-6，高峰时相为对照组的25倍；IL-6 McAb对胰岛培养上清中IL-6有很强的中和效应；斑点杂交结果显示LPS刺激后胰岛RNA中IL-6 mRNA含量明显高于对照组；人胚胎胰岛受LPS刺激后胰岛素分泌能力加强，胰高血糖素分泌能力明显降低。结论：免疫介质LPS可能影响胰岛的分泌功能及产生IL-6的能力。
关键词 人胚胎胰岛 IL-6 LPS
中国图书分类号 R335.6
Promotion of IL-6 production of human fetal
islets by LPS
SUN Rui,TIAN Zhi-Gang,YANG Gui-Zhen et al.Shandong Cancer Biotherapy Center,Shandong Academy of Medical Sciences,Jinan 250062
Abstract Objective:To study the effects of LPS on fetal islet function and IL-6 production in vitro.Methods：The islets from fetal pancrease was separated by collagenase type V (0.5 mg/ml)and cultured with or without LPS(10 μg/ml)in vitro.The supernatants of primary culture and second culture of human fetal islets for different days were assayed for IL-6,insulin,glucogon.Results:The results showedIL-6 secretion was siginificantly chanced and was maximal by 8th day after exposure to LPS and increased 145 folds (50 vs 7 284 mU/islet);After renewal of new mediual and restimulating with LPS,IL-6 secretion was increased 25 folds(47 vs 1 175 mU/islet);IL-6 McAb significantly neutralized the IL-6 activity of islet supernatants from each group,indicating that the IL-6 bioassay is specific;IL-6 mRNA in human fetal islet exposed to LPS is higher than control in dot hybridization;Insulin released to supernatants was increased (0.69～0.86 IU/islet)and glucagon secretion was decreased(1.80～0.18 pg/islet)after exposuring to LPS.Conclusion： Pancreatic islets may produce IL-6 and is promoted by LPS.The importance of IL-6 and LPS in regulating insulin and glucagon is under investigation.
Key words Human fetal islet IL-6 LPS
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