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急性肺损伤大鼠肺组织 和 2受体及 2受体mRNA表达的变化 |
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徐兴祥 孙耕耘 钱桂生
摘 要 目的：观察急性肺损伤(ALI)大鼠肺组织β、β2受体和β2受体mRNA含量的变化，探讨上述变化与ALI的关系及β2受体变化可能的分子机制。方法：静注内毒素复制大鼠ALI模型，肺组织β、β2受体及β2受体mRNA含量，分别用放射性配基结合分析法和斑点杂交技术测定。结果：静注内毒素后1、4、6 h，大鼠肺组织β和β2受体的最大结合容量(Bmax)均明显低于正常对照组(P＜0.01)，尤以β2受体为甚；静注内毒素后4及6小时，大鼠肺组织β2受体mRNA含量分别下降至正常对照组的76.4%±19.6%(P＜0.01)和52.8%±20.4%(P＜0.01)。结论：肺组织β受体数目的减少在ALI发生发展中可能起一定的作用，β2受体数目的减少可能与ALI关系更密切；β2受体mRNA含量的减少可能是ALI后一定阶段肺组织β2受体数目减少的原因之一。
主题词 内毒素类；肺；损伤；受体，肾上腺素能β；基因表达；大鼠
Changes of beta, beta-2-adrenergic receptor and beta-2-adrenergic receptor mRNA expression in lung tissue during endotoxin-induced acute lung injury
in rats
XU Xing－Xiang,SUN Geng－Yun,QIAN Gui-Sheng
Department of Respiratory Disease, Xing Qiao Hospital, Chongqing(400037)
Abstract AIM：To observe the changes of beta, beta-2-adrenergic receptors(β-AR,β2-AR) and beta-2-adrenergic receptor mRNA expression in lung tissue of rat with acute lung injurg (ALI), in order to explore the molecular mechanism of changes of beta-2-adrenergic receptor and the relation between above changes and ALI.METHODS：The model of ALI in rat was established by intravonous injection of E. coli endotoxin (ET). β-AR and β2-AR were measured by radioligand binding assay with ［3H］-dihydroalprenolol. The expression of β2-AR mRNA of lung tissue in rat was anlysed by dot blot hybridization. RESULTS：(1) The maximal capacity of the receptor (Bmax) of both β-AR and β2-AR in the lung tissue decreased significantly at the 1st, 4th and 6th hours after injection of ET. The density of β2-AR was much lower than that of β-AR.(2) The expression of β2-AR mRNA in lung tissue decreased to 76.4% and 52.8% of the level in control group at the 4th and 6th hours respectively after injection.CONCLUSIONS：(1)The decrease of density of β-AR in lung tissue may play some role in t[1] [2] [3] 下一页
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