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孤儿肽在自发性高血压大鼠和家兔心血管系统的分布 |
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米立国 魏英杰 丁金凤
【摘要】 目的 研究孤儿肽(orphanin FQ,OFQ)在自发性高血压大鼠和家兔心血管系统的分布。 方法 采用反转录 -聚合酶链反应(RT-PCR)法和免疫组织化学方法。 结果 RT-PCR的结果显示，OFQ的前体mRNA在自发性高血压大鼠的主动脉、肺动脉、肾动静脉均有较高的表达，其表达水平与脑组织表达水平相似，心房组织也有弱的表达，但在心室组织未见OFQ前体mRNA的表达。免疫组织化学结果显示，心肌细胞OFQ免疫反应阴性，心房内膜的内皮细胞和主动脉、肾动静脉的内皮细胞及血管平滑肌细胞均含有OFQ免疫反应阳性颗粒，此外在肾小球也观察到了OFQ反应阳性细胞。 结论 OFQ可能是对心血管系统和肾脏功能有一定调节作用的调节肽，但其具体的功能及调节机制尚待进一步研究。
【关键词】 孤儿肽 心血管系统 反转录-聚合酶链反应 免疫组织化学 家兔 自发性高血压大鼠
CARDIOVASCULAR DISTRIBUTION OF ORPHANIN FQ IN
SPONTANEOUSLY HYPERTENSIVE RAT AND RABBIT
Mi Liguo, Wei Yingjie, Ding Jinfeng△
(Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of
Medical Sciences Peking Union Medical College, Beijing)
【Abstract】 Objective To investigate the cardiovascular distribution of orphanin FQ (OFQ) and OFQ precursor mRNA in spontaneously hypertensive rat(SHR) and rabbit. Methods Reverse transcription-polymerase chain reacrtion(RT-PCR), and immunohistochemical method were used. Results The expression of OFQ precursor mRNA was detected from aorta, pulmonary artery, renal artery and vein of rat at a high level comparable with the amounts of brain, and a weak expression signal could also be observed in the atrium. The positive immunoreactive OFQ was detected in the tissues of aorta, atrium and renal of rabbit, as well as in smooth cells, endothelium and glomerulus. Conclusion These observations suggest that orphanin FQ might play a role in regulating the function of cardiovascular system and kidney, but the exact underlying mechanism needs further studying.
【Key words】 Orphanin FQ; Cardiovascular system; Reverse transcription-polymerase chain reaction; Immunohistochemistry; Rabbit;Spontaneously hypertensive rat
1995年Meunier［1］和Reinscheid［2］等分别自大鼠和猪的脑组织中分离出一种新的17肽，称为孤儿肽(orphanin FQ, OFQ)或痛敏肽(nociceptin)，OFQ来自一个较大的前体，与阿片肽有较高的同源性，并被证明为阿片受体样受体(opioid receptor-like receptor, ORL1)的内源性配体［1、2］，故很快引起人们的关注。OFQ前体mRNA在中枢神经系统有广泛的[1] [2] [3] 下一页
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