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乙型 丙型 庚型肝炎病毒多重感染研究 |
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杜绍财 陶其敏 常锦红 朱凌
【摘要】 目的 探讨庚型肝炎感染患者是否存在双重感染和多重感染。方
法 应用庚型肝炎病毒(HGV)NS3区逆转录聚合酶链反应技术检测了HGV系列稀释的
质控血清及Abbott GBV-C参比样品中HGV RNA，并对90例丙型肝炎病毒(HCV)RNA
阳性和12例乙型肝炎、丙型肝炎双重感染献血员进行了HGV RNA的检测。结果HGV系
列稀释质控血清10-1～10-5均为阳性；10-6为阴性。2份Abbott GBV-C样品均为
HGV RNA阳性。90例HCV RNA阳性样品中，8例HGV RNA阳性(17.8%)；12例乙、丙
双重感染者中4例(4/12)HGV RNA阳性。结论 不仅存在HCV及HGV双重感染，也存在
多重感染。
【关键词】 肝炎病毒，庚型 肝炎病毒，丙型 逆转录聚合酶链反应
Studies on Multiple Infection with Hepatitis B, C and G Viruses Du Shaocai, Tao
Qimin, Chang Jinghong, et al. Institute of Hepatology, People′s Hospital, Beijing Medical
University, Beijing 100044
【Abstract】 Objective To study if there exists superinfection and multiple infection
in the patients with hepatitis G virus (HCV) infection. Methods Reverse transcription
polymerase chain reaction (RT-PCR) technique in non-structural gene 3 (NS3) region of
HGV was used to detect HGV RNA in serially diluted quality control sera and HGV-C
reference panel of samples provided by Abbott Co. Ltd, in 90 cases with positive hepatitis
C virus (HCV) RNA, and 12 blood donors with super-infection of hepatitis B virus (HBV)
and hepatitis C virus (HCV). Results Serially diluted quality control sera showed positive
for HGV RNA at dilutions of 10-1to 10-5, but negative at dilutions of 10-6 to 10-8. HGV
RNA was positive in two samples of HGV-C serum provided by Abbott Co. HGV RNA
was positive in eight of the 90 cases with positive HCV RNA and in four of the 12 cases
with super-infection with HCV and HBV. Conclusion There are not only super-infection
of HBV and HCV, but also multiple infection.
【Key words】 Hepatitis G virus Hepatitis C virus Reverse transcription
polymerase chain reaction
自从1995年美国Simons等［１］及Linnen等［２］证实存在庚型肝炎病毒GBV-
C和HGV以来，引起了广大研究人员的极大重视。日本、西欧、澳大利亚、中国
等均有庚型肝炎病毒的存在。通过基因序列分析[1] [2] [3] 下一页
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