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宫内感染乙型肝炎病毒儿童接种乙型肝炎疫苗免疫失败与其相关细胞因子关系研究 |
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朱启镕于广军 吕晴 张公惠 顾新焕
【摘要】 目的 从相关细胞因子探讨宫内感染乙型肝炎病毒(HBV)导致乙肝疫苗免疫失败的机制。方法 对HBV宫内感染免疫失败儿童13例(无反应组)、宫内感染免疫成功儿童13例(有反应组)和正常免疫儿童21例(正常组)，测定血清可溶性白细胞介素2受体(sIL-2R)和分离外周血单个核细胞(PBMC)给予非特异性刺激植物血凝素(PHA)及特异性刺激乙型肝炎表面抗原(HBsAg)，进行体外细胞培养，用流式细胞仪检测PBMC白细胞介素受体（mIL-2R，或称CD25)，酶免夹心法检测PBMC受刺激后产生的IL-2、sIL-2R及IL-10的含量。结果 在PHA刺激下，三组对象PBMC表达mIL-2R的比例相近，差异无显著性，(F=0.107，P>0.05)；而当HBsAg刺激下正常组与无反应组比较(t=12.21，P<0.001)，有反应组与无反应组比较(t=7.43，P<0.001)，差异有极显著性。三组对象PBMC的mIL-2受体表达水平无反应组最低，其次为有反应组，正常组最高，三组间差异有显著性。sIL-2R水平相比无反应组低于有反应组和正常组，差异有极显著性。无反应组IL-2产生低下，而IL-10只见有反应组在外来刺激后升高。结论 宫内感染HBV导致免疫失败者经PHA非特异性刺激及HBsAg特异性刺激，IL-2分泌能力低下；HBsAg特异性刺激IL-2膜受体表达不足。可能是宫内感染HBV导致乙肝疫苗免疫失败的机制之一。宫内感染HBV接种乙肝疫苗免疫成功者，IL-10分泌能力增高，显示与其能产生抗体有关。
【关键词】 疾病传播，垂直 肝炎病毒，乙型 细胞活素类 肝炎，乙型
A study on the relationship between cytokines and failure of immunization with hepatitis B vaccine in children who had intrauterine HBV infection ZHU Qirong, YU Guangjun, LU Qing, et al. Children′s Hospital, Shanghai Medical University, Shanghai 200032
【Abstract】 Objective To study the immunoregulatory mechanisms of responsiveness and non-responsiveness to hepatitis B vaccine (HBVac) by analyzing the changes of IL-2/IL-2R system and IL-10 in children who had intrauterine hepatitis B virus (HBV) infection .Methods Forty-seven subjects, including 13 children who had intrauterine HBV infection and were not responsive to HBVac (non-responders), 13 children who had intrauterine HBV infection and were responsive to HBVac (responders) and 21 healthy children who were immunized against HBV (normal control).Peripheral blood mononuclear cells (PBMC) taken from the subjects were cultured in vitro and PBMC mIL-2R was determined by using flow cytometry; IL-2, sIL-2R and IL-10 contents were determined by using “sandwich” enzyme immunoassay.Results After specific stimulation with HBsAg, the expression levels of mIL-2R of the 3 groups showed significant difference, the lowest level was seen[1] [2] 下一页
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