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煤尘致肺泡巨噬细胞氧化损伤的细胞化学研究 |
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高俊玲 田艳霞 张宇新 崔建忠 孙树勋
【摘要】 目的 探讨煤尘致肺泡巨噬细胞(alveolar macrophages,AM)，损伤过程中是否存在着氧化损伤机制。 方法 应用细胞化学法显示煤尘作用于AM产生的和H2O2、并做定量测定及相关分析，以Mg2+-ATPase组织化学染色作为检测膜变化的指标。 结果 煤尘致AM产生的氧自由基较矽尘的少，同时细胞的形态结构和膜酶的损伤程度也较低。 结论 (1)应用抗氧化剂可减弱煤尘对AM所致的损伤，进而预防肺成纤维细胞增殖；(2)与矽尘相比，煤尘致AM氧化损伤的程度较弱；(3)形态学方法定位显示自由基较之自由基总体测定技术更能直观、具体地阐明细胞损伤的实际状况。
【关键词】 肺泡巨噬细胞；煤尘；自由基；损伤；细胞化学
【中图分类号】 Q24；R135.2 【文献标识码】 A
【文章编号】 0529-1356(2000)02-160
IN VITRO CYTOCHEMICAL STUDIES OF FREE RADICAL INJURY
ON RAT ALVEOLAR MACROPHAGES BY COAL DUSTS
GAO Jun-ling, TIAN Yan-xia, ZHANG Yu-xin,SUN Shu-xun
（Department of Histology and Embryology,North China Coal Medical College,）
CUI Jian-zhong
（Department of Nerosurgery,Tangshan Gongren Hospital,Tangshan 063000,China）
【Abstract】 Objective To analyze the relationship between the oxygen free radicals and morphological damage of alveolar macrophages(AMs)caused by coal dusts. Methods Using morphological technique to demonstrate the oxygen radicals.Moreover
,the cytochemical reaction slides of and Mg2+-ATPase were analyzed quantitatively by image analyzer,and the linear correlation tendency between the quantitative results of the and Mg2+-ATPase reaction was made. Results Like SiO2,a toxic mineral particle,exposure of AMs to coal dusts also resulted in production of and H2O2.They were localized on the plasma membrane,the membrane of the phogosome and/or pinosome,and the lysosomal membrane.There is a close relationship between AMs morphological and enzymatic damage and the production of oxygen free radicals. Conclusions (1)We can give antioxidant to the patients to terminal oxygenic damage in their bodies,further guard against proliferation of fibroblasts.(2)The degree of oxygen damage induced by coal dusts is lower than that by silica.(3)In situ evaluation of and H2O2 oxygen free radicals is superior to the other techniques in a sense.It is more convenient to directly obse[1] [2] 下一页
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