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大鼠去卵巢前后血清雌二醇浓度变化及其对输卵管粘膜上皮的影响 |
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刘爱军 罗善云 孟运莲
【摘要】 目的 研究哺乳动物血清雌二醇与输卵管粘膜上皮细胞高度之间的相关性。 方法 据阴道涂片检查将动物分为：动情间期(DE)组，动情前期(PE)组，动情期(E)组，动情后期(ME)组和去卵巢(OVX)组，用放射免疫法检测各组大鼠血清雌二醇浓度，同时在光镜下对输卵管壶腹部、峡部的形态结构进行测量分析。 结果 大鼠血清雌二醇浓度依次为：动情期组＞动情前期组＞动情后期组＞动情间期组＞去卵巢组，各组间差异均有显著性；输卵管粘膜上皮高度在动情周期中变化为：动情期组＞动情前期组＞动情间期组＞动情后期组；去卵巢后，输卵管粘膜上皮萎缩。 结论 上述两项检测结果经回归分析发现，从动情间期至动情前期再至动情期两者呈正相关。
【关键词】 雌二醇；输卵管；动情周期；去卵巢
【中图分类号】 R329.1 【文献标识码】 A
【文章编号】 0529-1356(2000)02-164
CHANGE OF ESTRADIOL LEVEL IN SERUM AND ITS INFLUENCE ON THE OVIDUCTAL EPITHELIA BEFORE AND AFTER OVARIECTOMY IN RAT
LIU Ai-jun,LUO Shan-yun,MENG Yun-lian
（Department of Histology and Embryology,Hubei Medical University,
Wuhan 430071,China）
【Abstract】 Objective This study was undertaken to find the relationship between the estradiol level in serum and the epithelial height of the oviduct in rats. Methods The animals were devided into five groups,including diestrus group(DE),prosetrus group(PE),estrus group(E),metaestrus group(ME),and ovariectomy group(OVX).The estradiol level in serum was detected by radio immunoassay during estrous cycle and after ovariotomy in rats.At the same time,the morphological variations of the ampulla and ithmus of the oviducts were observed and measured by light microscopy. Results The serum levels of estradiol were E＞PE＞ME＞DE＞OVX.The differences between the two groups were significant.The height of the oviductal epithelia was E＞PE＞DE＞ME.After ovariotomy,the epithelia of the oviduct were atrophied and regressive. Conclusion The analysis of regression showed that there was a linear regression between the estradiol level in serum and the height of the oviductal epithelia.
【Key words】 Estradiol; Oviduct; Estrous cycle; Ovariectomy
现已知，输卵管作为卵巢激素的靶器官，随雌、孕激素的周期性变化，其结构和功能亦发生相应变化，迄今，尚未见两者间数量变化关系的研究报道。本研究通过对同一群体大鼠血清雌二醇浓度消长与输卵管粘膜上皮高度的检测，探讨两者的相关性。
材料和方法
1. 实验动物及分组
用成年SD雌鼠25只，体重200～250g，根据阴道细胞学检查将动物随机分为5组；动情间期组[1] [2] 下一页
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