|
氯胺酮对兔肠缺血再灌注肺的保护作用 |
| |
氯胺酮对兔肠缺血再灌注肺的保护作用 摘要 目的:研究氯胺酮对兔肠缺血再灌注肺的保护作用。方法：选择健康家兔24只,利用肠系膜上动脉钳夹法制备肠缺血再灌注模型,随机分为正常对照组、缺血再灌注组、氯胺酮处理组。氯胺酮处理组在钳夹肠系膜上动脉前和开放前予KTM2mg.kg-1静注,缺血再灌注组予等量生理盐水静注。分别测定不同时段的肿瘤坏死因子活性,并于再灌注4小时后处死家兔,开胸取肺,测定肺组织匀浆肿瘤坏死因子活性,并做病理组织检查。 结果：缺血后1h,再灌注后1h、2h、4h血浆肿瘤坏死因子活性均较缺血前升高(P<0.05),再灌注1h达到高峰,以后逐渐下降,而氯胺酮处理组与缺血再灌注组比较缺血后1h,再灌注后1h、2h、4h血浆肿瘤坏死因子活性降低(P<0.05)。肺匀浆肿瘤坏死因子活性,氯胺酮处理组较缺血再灌注组低(P<0.05)。肺组织病理,缺血再灌注组与正常对照组比较有急性肺损伤的改变,而氯胺酮处理组与正常对照组比较改变不明显。 结论：氯胺酮对兔肠缺血再灌注肺有保护作用。 关键词 氯胺酮 肠缺血再灌注 肿瘤坏死因子Abstract Objective：To investigate the protect effect of ketamine on lung after interstinal ischmia reperfusion in rabbits. Methods:Twenty-four rabbits were divided into three groups randomly, i.e. normal contral group, inchemia reperfusion group and ketamine group. Rabbits received an IV bolus of either ketamine 2mg/kg(ketamine group)or an equal amount of isotonic sodium chloride solution(inchemia reperfusion group). Plasma TNF activity in before interstinal ischmia(T0), 1h after interstinal ischmia(T1),1h after reperfusion(T2),2h after reperfusion(T3),4h after reperfusion(T4), was observed. The TNF activity and pathologic changes in lung was observed. Results:The plasma of TNF activity at T2、T3、T4 in two groups were higher than those at T0(P<0.05). The plasma of TNF activity in ketamine group were lower than those in inchemia reperfusion group(P<0.05).Lung pathological examination showed that actue lung injury was more predominant in ischemia reperfusion group than that of normal control group but lung pathological examination was not obviously changes in ketamine group than normal control group. Conclusion:It suggests that the treatment with ketamine can protect lung after intetinal ischemia reperfusion.Key Words ketamine Inchemia reperfusion Rab[1] [2] [3] 下一页
上一个医学论文： 浅析肺栓塞的误诊原因
下一个医学论文： MSCT引导经胸细针穿刺对肺内小肿块的诊断
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 内科论文 >> 正文