【摘要】 目的 探讨幽门螺杆菌(Hp)vacA、cagA基因型与胃疾病的关系。方法 选取105例胃疾病病人，包括慢性浅表性胃炎(CSG)45例，慢性萎缩性胃炎(CAG)48例，胃癌(GC)12例。于胃窦处取3块胃黏膜，分别进行快速尿素酶反应、 病理检查和聚合酶链反应(PCR)。提取胃黏膜基因组DNA，用6对引物检测 Hp vacA、cagA 基因的表达。结果 快速尿素酶反应、病理检查均阳性的标本57例，Hp的阳性率为54.2%(57/105)。Hp vacA s1/m2、s1/m1、s2/m1、s2/m2 的阳性率分别为 53%(30/57)、18%(10/57)、8%(5/57)、21%(12/57);Hp cagA的阳性率为89%(51/57)。vacA、cagA基因型在CSG、CAG 和GC之间差异无显著性(P>0.05)。结论 Hp菌株的优势基因亚型为Hp cagA、vacA s1/m2;Hp vacA、cagA基因与特定胃疾病间无显著相关性，不能预示Hp感染的临床结果
【关键词】 胃疾病;螺杆菌,幽门;胃黏膜;vacA基因;cagA基因;聚合酶链反应
RELATIONSHIP BETWEEN GENOTYPES OF HELICOBACTER PYLORI vacA AND cagA AND GASTRIC DISEASES
ZHANG FENGJUAN, JIANG YUEPING, ZHAO QINGXI
Department of Gastroenterology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China) ;
[ABSTRACT] Objective To investigate the relationship between vacA and cagA genotypes of Helicobacter pylori (Hp) and gastric diseases. Methods This study consisted of 105 patients with various gastric diseases including chronic superficial gastritis (CSG, 45 cases), chronic atrophic gastritis (CAG, 48), and gastric cancer (GC, 12). Three pieces of antrum mucosa were obtained from each patient. Rapid urease test, histological examination and polymerase chain reaction (PCR) were carried out in the specimen. DNA was extracted from the specimen, and six pairs of primers were used to detect the gene expression of vacA and cagA. Results Fiftyseven patients showed positive results in rapid urease test and histological examination, in which, the positive rate of Hp infection was 54.2% (57/105). The positive expression rates of vacA s1/m2, s1/m1, s2/m1, s2/m2 were 53% (30/57), 18% (10/57), 8%(5/57) and 21% (12/57), respectively. The expression rate of cagA was 89% (51/57). There was no significant differences in the expression of vacA, cagA among CSG, CAG and GC groups (P>0.05). Conclusion CagA and vacA s1/m2 were the dominant genotypes of Helicobacter pylori. There was no
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