|
雌激素与孕激素对卵巢癌耐药细胞系OVCAR |
| |
孟君艳 尤宗兵 郭燕燕
【摘要】 目的 研究雌、孕激素对卵巢癌耐药细胞系OVCAR-3生长及对阿霉素（ADM）耐药的影响。方法 用四甲基偶氮唑蓝（MTT）快速比色法， 计算细胞生存率，以比较不同浓度雌、孕激素对细胞生长的影响，及雌、孕激素与异搏定对卵巢癌耐药细胞系OVCAR-3耐药性的影响。结果 高浓度的苯甲酸雌二醇（E2，10.00～100.00 μmol/L)和安宫黄体酮（MPA，100.00 μmol/L)可抑制OVCAR-3细胞的生长，且二者联合抑制作用增强；而较低浓度的E2(0.01～1.00 μmol/L)和MPA(0.01～10.00 μmol/L)对该细胞的生长无明显影响；异搏定可增加ADM对OVCAR-3细胞的抑制作用，即逆转该细胞对ADM 的耐药；E2或MPA均可增加ADM对OVCAR-3细胞的抑制作用，即逆转该细胞对ADM的耐药，且其作用强于异搏定。 结论 超生理浓度的雌、孕激素可抑制OVCAR-3细胞的生长，而较低浓度的雌、孕激素对其生长无影响；雌、孕激素可逆转OVCAR-3细胞对ADM的耐药，且作用强于异搏定，可望成为临床化疗的辅助用药。
【关键词】 雌激素类 甲羟孕酮卵巢肿瘤 肿瘤细胞，培养的 抗药性，肿瘤
Effects of Estradiol and Medroxyprogesterone on the Growth and Doxorubin-resistance of Drug-resistant
Human Epithelial Ovarian Cancer Cell Line OVCAR-3
MENG Junyan*, YOU Zongbing, GUO Yanyan. *
711 Hospital of China Areospace Corporation, Beijing 100034
【Abstract】 Objective To study the effects of estradiol (E2) and medroxyprogesterone acetate (MPA) on the growth of drug resistant human epithelial ovarian cancer cell line. Methods OVCAR-3 cells, treated by different concentrations of E2, MPA and verapamil, were examined for their growth inhibitions with methyl thiazolyl tetrazolium (MTT, a tetrazolium dye) rapid photocolorimetric assay. Results OVCAR-3 cell growth was inhibited by E2 at high concentrations (10.00～100.00 μmol/L)and by MPA at 100.00 μmol/L, while lower concentrations of E2(0.01～1.00 μmol/L) and MPA(0.01～10.00 μmol/L) had no effect on OVCAR-3 cell growth, neither stimulation nor inhibition. The effect of E2 combined with MPA was more obvious than either one used separately. E2 and MPA in combination with doxorubin showed a synergistic inhibition effect on OVCAR-3 cell growth, which suggested that both of them could reverse the doxorubin resistance of OVCAR-3 cell line. The inhibition effect of Verapamil combined with doxorubin was obviously weaker than that of E2 and MPA. Conclusions E2 and MPA could reverse the do[1] [2] 下一页
上一个医学论文： Norplant皮下埋植避孕法使用七年临床观察
下一个医学论文： 子宫内膜样腺癌血管增生与p53蛋白表达的关系
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 妇产科论文 >> 正文