【摘要】目的 探讨孕妇血浆中胎儿游离DNA及抗DNA抗体与子痫前期的相关性。方法 以Y染色体上性别决定基因(SRY基因)为基因标志, 采用荧光定量PCR技术检测分娩后证实为男婴的34例子痫前期孕妇(观察组)和42例正常孕妇(对照组)血浆中胎儿游离DNA(fDNA)水平，采用ELISA法检测抗ss-DNA及抗ds-DNA抗体水平。 结果 观察组fDNA水平为(233.69±125.49)，对照组为(67.83±43.70)，两组比较，差异有统计学意义(P<0.01)。观察组抗ss-DNA抗体水平为(0.110±0.141)，对照组为(0.067±0.074)，两组比较，差异有统计学意义(P<0.05)。观察组抗ds-DNA抗体水平为(0.0267±0.0105)，对照组为(0.0230±0.0102)，两组比较，无统计学意义(P>0.05)。fDNA与抗ss-DNA抗体无相关性(r=0.1275,P>0.05)，与抗ds-DNA抗体无相关性(r=0.245,P>0.05)。 结论 监测孕妇血浆fDNA变化可作为预测子痫前期及诊断病情程度的一个指标。fDNA升高并非由抗DNA抗体所致。
【关键词】 DNA 抗DNA抗体 子痫前期
【Abstract】 Objective To explore the relationship of fDNA and antiDNA antibody in pregnant women with preeclampsia. Methods SRY gene is used as gene marker. Thirty-four cases of pregnant women with preeclampsia and forty-two cases normal pregnant women were selected. Both groups delivered male babies. The fDNA level in the plasma of the women were quantitated by PCR. Antibody levels of ss-DNA and ds-DNA were detected by ELISA. Result Mean fDNA level in test groups (233.69±125.49)was significantly higher than that (67.83±43.70)in control group (p<0.01); Plasma level of antiss-DNA antibody levels in test group (0.110±0.141) was significantly higher than that (0.067±0.074) in control group (p<0.05); however, plasma level of antids-DNA antibody had no significant difference (P>0.05). No correralation was found between fetal DNA levels and antiss-DNA antibody or antids-DNA antibody in preelampsia group (r=0.1275,P>0.05). Conclusion Detection of the variation of fDNA in the plasma of the pregnant women may help to forecast and diagnose preeclampsia, the rise of fDNA is not caused by antiDNA antibody.
【Key words】DNA AntiDNA antibody Preeclampsia
妊娠期高血压疾病是妊娠期特有的疾病，是导致孕妇和围生儿死亡的主要原因，目前其病因及发病机制仍未明确。近年来研究发现孕妇血浆中存在着胎儿游离DNA，妊娠合并子痫前期妇女血浆中胎儿游离DNA含量明显增高，且与疾病的严重程度相关。本研究采用荧光定量聚合酶链反应(PCR)方法对不同程度妊娠期高血压疾病患者外周血浆进行SRY基因检测，以探讨孕妇血浆中胎儿游离DNA与妊娠期高血压疾病的关系，现将结果报道如下。
1 临床资料
1.1 一般资料
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