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豆蔻酰佛波醇乙酯与 |
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赵霞 魏于全 彭芝兰 曹泽毅
【摘要】 目的 探讨豆蔻酰佛波醇乙酯(PMA)和γ-干扰素(γ-IFN )在卵巢癌及其他肿瘤治疗中的意义。方法 用PMA+γ-IFN处理的卵巢癌细胞及其他肿瘤细胞用流式细胞仪检测免疫球蛋白超家族糖蛋白的共刺激分子B7-1、主要组织相容抗原(MHC-Ⅰ、MHC-Ⅱ）的表达情况。并采用51铬(51Cr) 4小时释放试验及［3H］胸腺嘧啶渗入法了解B7-1的表达对细胞毒性T淋巴细胞(CTL)杀伤活性和对T淋巴细胞增殖的诱导情况。结果 预先用PMA处理，后用γ-IFN处理的肿瘤细胞，能诱导B7-1表达阳性的伯基特淋巴瘤细胞株或转染 B7-1基因的卵巢粘液性囊腺癌细胞的B7-1表达增强，能诱导无 B7-1、MHC-Ⅰ、MHC-Ⅱ表达的卵巢癌细胞株及其他肿瘤细胞株表达 B7-1及MHC-Ⅰ、MHC-Ⅱ，而单独用γ-IFN则不能诱导 B7-1表达。PMA和γ-IFN的这种协同效应能被蛋白激酶抑制剂1-( 5 -磺酰基)异喹啉-2 -甲基哌嗪盐酸盐(H7)阻断。被诱导表达的 B7-1能激活CTL的杀伤活性，并能增强T淋巴细胞的增殖能力。结论 γ-IFN诱导的B7-1的表达可能依赖于PMA预先激活蛋白激酶C。诱导B7-1等的表达对治疗肿瘤有一定的价值。
【关键词】 干扰素Ⅱ型 抗原，CD80 T淋巴细胞 淋巴细胞转化 十四酰佛波乙酯
Phorbol-12-myristate-13-acetate and Interferon-γ Synergistic Induction of B7-1 Expression in Ovary Carcinoma Cells and Immunological Value
ZHAO Xia, WEI Yuquan, PENG Zhilan, et al.
Department of Obstetrics and Gynecology, Second University Hospital, West China University of Medical Sciences, Chengdu 610041
【Abstract】 Objective To investigate the therapeutic potential of phorbol-12-myristate-13-acetate(PMA) and interferon-γ(IFN-γ) by induction of expression of adhesion molecule in mucious cystadenocarcinoma of ovary(MCAS) and other tumor cells. Methods Expression of costimulatory signals(B7-1), major histocompatibility complex(MHC-Ⅰ、MHC-Ⅱ) was analysed with flow cytometry. Cytotoxic T lymphocytes(CTL) killing activity and T cell proliferation induced with expression of B7-1 were evaluated by 4-h 51Cr release assay and standard3 HTdR incorporation and scintitation counting. Results Pretreatment with PMA and then treated with IFN-γ was able to enhance B7-1 expresssion on the tumor cell lines with the constitutive B7-1 expression or on B7-1-transfected tumor cells and to induce B7-1 expression on MCAS and some tumor cell lines without constitutive B7-1 expression. However, treament with IFN-γ alone failed to induce B7-1 expression on the tum[1] [2] [3] 下一页
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