|
调压益心胶囊中钩藤的薄层层析鉴别 |
| |
熊学敏 康明 汤益明 廖培盛 熊毅敏 张举玲
提要 目的：用薄层层析和紫外分析方法对调压益心胶 囊中所含的钩藤成份进行分析鉴别。方法：以中药钩藤对照药材作为对照品，以 0.5%CMC-Na、硅胶G铺板，在氯仿－乙酸乙酯－乙醇(4.5∶4∶1.5)；苯－氯仿－丙酮－氨水 (3∶5∶2∶0.5)二个系统中分别展开后喷碘化铋钾、碘、碘化钾试剂显色。结果： 在同一薄层色谱中， 和药材对照品相同的位置上，供试品显清晰分离良好的斑点，阴性对照品无此斑点。结论： 该方法具有定性检查的鉴别意义，为制定调压益心胶囊质量标准提供了实验方法和依据。
关键词 调压益心胶囊； 华钩藤； 薄层层析； 鉴别
TLC Identification of Ramulus Uncaria Cum Uncis in The Tiaoya-yixin Capsule
Xiong Xuemin, Kang Ming, Tang Yiming, et al
Jiangxi Provincial Institute of Traditional Chinese Medicine and Materi a Medica, Nanchang 330077
Abstract Objective: The composition of Ramulus Uncari a cum uncis in Tiaoya-yixin capsule was identified with TLC and UV.Method: Ramul us Uncaria Cum Uncis based on herbs, was used as control herb. Silicon G bed of 0.5%CMC-Na took on colour after spraying iodide postassium bismuth, and iodine-p ostassium iodide repectively on group one of chroloform acetyacetic ether-alcoho l(4.5∶4∶1.5) and group two of benzene-chloroform-acetone-ammonia water(3∶5∶2 ∶0.5). Result: In the same chromatogram and on the same place of control herb, subject herb appeared well-separated spot, but no spots in negative control herb . Conclusion: This method could identify qualitive determination, present experi mental approaches and evidences leading to the quality regulations of Tiaoya-yix in capsule.
Key words Tiaoya-yixin capsule; Uncaria sinensis (Oliv) Havil; TLC; Indentification
调压益心胶囊是由黄芪、丹参、川芎、党参、钩藤、汉防已、生地、山茱萸等中药组成，临 床上用于治疗高血压伴有心室肥厚(LVH)的患者。钩藤系主要原料之一，为茜草科植物钩藤U ncaria rhynchophylla (Miq) Jacks,大叶钩藤Uncaria macrophylla wall,毛钩藤Uncaria hirsuta Havil,华钩藤Uncaria Sinensis (Oliv) Havil或无柄果钩藤Uncaria Sessilifruc tus Roxb的干燥钩茎枝。具有清热平肝、熄风定惊、降血压等功效［1］。钩藤主要 含吲哚类生物碱，它们分别为钩藤碱(Phynchophylline)、异钩藤碱(Isohlynchop hylline)。此二种生物碱为降血压的有效成份。另外还含有去氢钩藤碱(Corynoxein e)、异去氢钩藤碱(Isocroynoxeine)、毛帽柱木碱(Hirsutine)、去氢毛帽柱木碱(Hirsutei ne)、兜花木碱(Corynantheine)及二氢兜花木碱(Dihyolrocrynantheine)［2］。本 项研究所用钩藤为华钩藤Uncaria sine[1] [2] 下一页
上一个医学论文： 注意菟丝子掺伪
下一个医学论文： 气相色谱检测复方丹参片中有关物质
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 中医论文 >> 正文