|
脊髓缺血损伤模型及行为学的实验研究 |
| |
伍亚民 王正国 朱佩芳 廖维宏
摘 要：目的 制作一种新的家兔脊髓缺血损伤模型，观察其行为功能改变。 方法 以选择性阻断腰动脉制作腰髓缺血损伤模型，32只家兔随机均分为对照组，缺血30，60及90 min组。采用Jacobs法对家兔后肢功能评级，Reuter法对脊髓感觉运动反射功能评分。 结果 再灌注1 d时，缺血30 min家兔轻瘫，缺血60，90 min家兔全部(8/8)发生完全性截瘫。Reuter分值变化趋势为缺血90 min组>缺血60 min组>缺血30 min组>对照组。 结论 选择性阻断腰动脉血流30，60，90 min可以模拟出轻、中、重不同程度局部脊髓缺血再灌注损伤。本模型是一种较理想的脊髓缺血损伤模型。
关键词：兔； 脊髓损伤； 局部缺血； 行为
Experimental Study on ischemic injury model of spinal cord and behavior
WU Yamin WANG Zhengguo ZHU Peifang, et al.
（Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China）
Abstract：Objective To make a new ischemic injury model of spinal cord and investigate function changes of behavior in rabbits. Methods The ischemic injury model of spinal cord was created by the selective occlusion of lumbar arteries in 32 rabbits，which were randomly and equally divided into four groups, i.e. control group, ischemia for 30 minutes, ischemia for 60 minutes and ischemia for 90 minutes. The function of hind extremities was evaluated by the Jacobs method; and the sensory, motor and reflex functions of spinal cord were scored through the Reuter method. Results A mild paralysis was noted in the ischemia for 30 minutes group from one day perfusion, but the complete paralysis of all rabbits happened in the two groups of ischemia for 60 minutes and ischemia for 90 minutes. The Reuter s score in the order from the highest to the lowest was ischemia for 90 minutes, ischemia for 60 minutes,ischemia for 30 minutes and control group. Conclusions Mild, moderate and severe reperfusion injury of regional spinal cord ischemia can be simulated by the selective occlusion of lumbar artery for 30 minutes, 60 minutes and 90 minutes respectively. It is a very good model of ischemic injury in spinal cord.
Key words：Rabbits; Spinal cord injuries; Ischemia； Behavior▲
脊髓继发性损伤最基本的机制是“血管机制”，即脊髓缺血缺氧在脊髓继[1] [2] 下一页
上一个医学论文： 血小板在脂肪栓塞综合征发病及诊断中的作用
下一个医学论文： 脂多糖刺激肺血管内巨噬细胞环氧合酶
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 外科论文 >> 正文