|
食管癌低表达cDNA片段C6 |
| |
吴孔明 许智雄 王明荣* 徐昕 韩亚玲 蔡岩 王瑞林 孙燕 吴旻
【摘要】 目的 克隆人食管癌发生相关的基因。方法 用mRNA差异显示技术分离、克隆食管癌组织中不表达或低表达的cDNA片段，再通过Northern blot、dot blot和RT-PCR证实。结果 获得280bp的cDNA片段，命名为C6-2A。与GenBank基因数据库比较，未发现C6-2A与任何已知基因有同源性。查询EST数据库，发现C6-2A与ne27b03,s1NCI-CGAP-C03人cDNA克隆898541及人卵巢肿瘤cDNA克隆755196等高度同源。Northern blot结果显示6/6例食管癌组织表达丧失或低表达，dot blot分析表明7/8例食管癌组织低表达，RT-PCR显示食管癌细胞系EC109、EC8712、EC9706和肺腺癌细胞系GLC-82极弱表达，17/20例食管癌组织表达丧失或低表达，胎儿食管、皮肤、大脑、胎盘较高表达，胎儿胃、肝较弱表达，胎儿心、小肠、肾不表达。结论 在食管癌细胞系和食管癌组织高频率的不表达或低表达提示，C6-2A很可能与食管癌的发生、发展有关。
【关键词】 食管癌 mRNA差异显示 基因表达 cDNA
Cloning and expression analyses of down-regulated
cDNA C6-2A in human esophageal cancer
WU Kongming, XU Zhixiong, WANG Mingrong*, XU Xin, HAN Yaling,
CAI Yan, WANG Ruilin, SUN Yan, WU Min.
* National Laboratory of Molecular Oncology, Department of Cell Biology, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical college, Beijing 100021 P.R.China. E-mail：[email protected]
【Abstract】 Objective To clone genes associated with the genesis of human esophageal cancer.Methods Identifying missing or low expressing cDNAs in human esophageal cancer tissues by mRNA differential display and examining its mRNA expression in 4 human cancer cell lines, 9 fetal tissues and other matched esophageal cancer tissues by Northern blot, dot blot and RT-PCR.Results One cDNA fragement named C6-2A, was cloned and sequenced. There was no identical sequence with C6-2A in BLASTN database; but in querying Genbank EST, the authors found that C6-2A was identical with ne27b03.s1NCI-CGAP-C03 humans sapiens cDNA clone IMAGE:898541 3′ and zv30g07.r1 Soares ovary tumor NbHOT homo sapiens cDNA clone 755196.6/6 esophageal cancer tissues in Northern blot and 7/8 in dot blot did not or slightly express C6-2A. RT-PCR analysis showed that C6-2A was expressed much lower in 17/20 espohageal cancer tissues than adjacent microscopically normal mucosa, highly [1] [2] [3] 下一页
上一个医学论文： 男性服刑罪犯的个性特征研究
下一个医学论文： 一个母系遗传非综合征耳聋大家系及mtDNA 12SrRNA基因突变研究
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文