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金属硫蛋白对 射线和丝裂霉素C致遗传损伤的拮抗作用 |
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薛宏伟 郝福英 魏雪涛 文小青 蒋 睿 林慰慈
摘要 目的 探讨兔肝锌金属硫蛋白（Zn-MT）对γ射线和丝裂霉素C(MMC)所致遗传损伤的拮抗作用。方法 应用体内外微核试验和单细胞凝胶电泳法分别检测γ射线照射或MMC处理前后给以Zn-MT对微核形成或DNA链断裂的影响。结果 664μg/kg剂量Zn-MT对5Gyγ射线诱发的小鼠骨髓微核形成有抑制作用。10、50μg/mlZn-MT能拮抗1和3Gyγ射线及0.3μg/mlMMC诱发的g12细胞双核微核细胞率升高,50μg/mlZn-MT能使1Gyγ射线诱发的链断裂减少。结论 兔肝Zn-MT对γ射线和MMC所致遗传损伤有一定拮抗作用。
关键词 金属硫蛋白 γ射线 丝裂霉素C DNA损伤
Antagonistic Effects of Metallothionein on Genetic Damage Caused by γ-Rays and Mitomycin C
XUE Hongwei*, HAO Fuying, WEI Xuetao, et al.
*Department of Health Toxicology, Beijing Medical University, Beijing 100083
Abstract Objective To study the antagonistic effects of metallothionein （Zn-MT）on genetic damage caused by γ-rays and mitomycin C （MMC）in the liver of rabbits. Methods Micronucleus test and DNA strand breaks were determined in vitro and in vivo with single-cell gel electrophoresis assay to observe the effects of Zn-MT on the micronucleus formation and cleavage of DNA chain before and after γ-rays radiation and MMC treatment. Results A dose of 664 μg/kg of Zn-MT could inhibit micronucleus formation in the bone marrow of the mice induced by 5 Gy γ-rays radiation（P<0.05）. Concentrations of 10 and 50 μg/ml Zn-MT could antagonize the increase in bimicronucleus frequency in g12 cells induced by 1 and 3 Gy γ-rays radiation and 0.3 μg/ml MMC treatment （P<0.05 or P<0.01）. A dose of 50 μg/ml Zn-MT could reduce the DNA single strand breaks caused by 1 Gy γ-rays radiation, but without statistical significance （P>0.05）. Conclusion Zn-MT could antagonize the genetic damage to certain extent in the liver of the rabbits caused by γ-rays radiation and MMC treatment.
Key words Metallothionein Gamma rays Mitomycin C DNA damage
金属硫蛋白(MT)是一类富含半胱氨酸的金属结合蛋白质,对电离辐射和自由基造成的损伤有一定的保护作用［1，2］。我们为研究锌诱导的兔肝金属硫蛋白（Zn-MT）对 60 Co-γ射线和丝裂霉素C(MMC)诱发的体内、体外微核[1] [2] 下一页
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