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中国人群D20S161基因座的遗传多态性 |
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李英碧 吴谨 金泽明 侯一平*
【摘要】 目的 了解我国南北汉族、藏族、白族、壮族、蒙古族群体的遗传亲缘关系，获得我国自然人群中D20S161基因座的群体遗传数据。方法 753份EDTA抗凝血样分别采自成都、广州、吉林、拉萨、大理、南宁、海拉尔的无血缘关系个体，Chelex法提取DNA，PCR扩增样本DNA后，聚丙烯酰胺凝胶水平电泳分型。结果 在中国7个群体中，6个群体在D20S161基因座存在7个等位基因，1个群体存在6个等位基因；观察到的基因型为16～25种。等位基因频率为D20S161*16：0.0220～0.0657，D20S161*17：0.1429～0.2947，D20S161*18：0.3237～0.4835，D20S161*19：0.0909～0.1850，D20S161*20：0.1263～0.1786，D20S161*21：0.0421～0.1010，D20S161*22：0～0.0250。7个群体的基因型分布均符合Hardy-Weinberg平衡。结论 中国人群中，D20S161基因座存在7个常见等位基因，以D20S161*18频率为最高。基因频率分布在中国不同群体之间无显著性差异(χ2＝57.9342，P＞0.01)。对于人类群体遗传学和法医遗传学，D20S161是一个十分有用的遗传标记。
【关键词】 短串联重复序列 D20S161基因座 聚合酶链反应 遗传多态性
GENETIC POLYMORPHISM AT D20S161 LOCUS IN CHINESE POPULATIONS Li Yingbi, Wu Jin, Jin Zeming, Hou Yiping*.* Department of Forensic Haemogenetics, West China University of Medical Sciences, Chengdu 610041 P.R.China
【Abstract】 Objective To understand the genetic relationship between different populations in China and to obtain the preliminary data on the genetic polymorphisms of D20S161 in seven populations in China. Methods EDTA-blood specimens were collected from 753 healthy unrelated individuals in seven Chinese populations, including Chengdu(Han), Guangzhou(Han), Jilin(Han), Lasa(Tibetans), Dali(Bai), Nanning (Zhuang) and Hailaer (Menggu), respectively. DNA was extracted with Chelex method. The DNA samples were amplified by PCR technique, and the PAGE horizontal electrophoresis was used for typing the PCR products. Results Seven alleles at D20S161 locus were observed in six populations, while six alleles at D20S161 were observed in one population in Dali(Bai). The numbers of genotypes observed were 25,19,21,19,16,20 and 22 for Chengdu(Han), Guangzhou(Han), Jilin(Han), Lasa(Tibetans), Dali(Bai),Nanning(Zhuang) and Hailaer(Menggu), respectively. The allele frequencies were D20S161*16:0.0220～0.0657，D20S161*17:0.1429～0.2947，D20S161*18:0.3237～0.4835，D20S161*19:0.0909～0.1850，D20S161*20:0.1263～0.1786，D20S161*21:0.0[1] [2] [3] 下一页
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