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高胆固醇诱发 Oddi括约肌功能紊乱实验研究 |
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魏经国 杜凡 王耀程 王玮
［摘 要］ 目的：探讨高胆固醇血症胆管 Oddi括约肌功能改变特征及病理组织基础。方法：纯种新西兰兔24只，随机分为3组，喂饲胆固醇饲料制作高胆固醇血症模型。采用胆管造影，单腔管测压，一氧化氮合酶定量分析等方法，研究胆管 Oddi括约肌影像、功能及病理组织学的特征。结果：高胆固醇血症胆管Oddi括约肌近侧低压段基础压力实验Ⅰ组和Ⅱ组（20.9±6.1) mmHg，(25.6±9.1) mmHg显著高于对照组（11.7±2.8) mmHg，(P＜0.01， P＜0.01)， 远端高压“阀门”段的基础压力实验Ⅰ、Ⅱ组（138.4±45.5) mmHg，(144.5± 40.4) mmHg与对照组（69.3±9.8) mmHg比较，差异非常显著（P＜0.01，P＜0.01)。胆囊容积增大，实验Ⅰ、Ⅱ组（3.4 ±1.0) mL, (3.9 ±1.9) mL明显大于对照组（2.0±1.9) mL(P＜0.05， P＜0.01)。一氧化氮合酶定量分析各组间无明显差异(P＞0.05 )。实验组胆管 Oddi括约肌细胞体积增大，细胞膜扭曲呈波浪状。细肌丝疏松，排列紊乱，密体分布不均，形态大小不一致，长轴与细肌丝纵轴成角。线粒体肿胀，嵴消失，聚集於细胞核一端。染色质浓集於细胞核边缘。结论：高胆固醇可损害胆管Oddi括约肌的舒张功能，诱发Oddi括约肌功能紊乱，导致胆管Oddi括约肌排胆阻力增加而致胆囊淤胆。
［主题词］ 胆固醇；奥狄氏括约肌；显微镜检查，电子
［中图分类号］ R575.7 ［文献标识码］ A
［文章编号］ 1000-4718(2000)05-0446-05
An experimental study of hypercholesterolemia-induced disorder of Oddi's sphincter
WEI Jing-guo, DU Fan, WANG Yao-cheng, WANG Wei
(Department of Radiology, Tangdu Hospital, The Fourth Military Medical University, Xian 710038,China)
［Abstract］ AIM：To investigate the functional changes and histopathological basis of Oddi's sphincter under hypercholesterolemia. METHODS:Twenty-four New Zealand rabbits were equally divided into three groups randomly. Experimental groups I and II fed with cholesterol-added forage for 4 and 8 weeks respectively before sacrificed. Images, functions and histopathological characteristics of Oddi's sphincters of experimental groups were studied and compared with control group by cholangiography, catheter manometry, and quantitative analysis of nitric oxide synthase. RESULTS:The basal pressures of proximal low-pressure segment of Oddi's sphincter of both experimental groups I and II (20.9±6.1 mmHg, 25.6±9.1 mmHg, respectively) were higher than those of control group significantly (11.7±2.8 mmHg, P＜0.01, P＜0.01). The basal pressures of distal high-pressur[1] [2] [3] 下一页
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