|
慢性应激对糖尿病倾向鼠几种组织抗氧化酶影响的差异及其意义 |
| |
刘幼硕 胡敏 姚树桥 蔡伟雄 戴晓阳 龚耀先
摘 要：目的：研究慢性应激对正常及糖尿病倾向小鼠各组织抗氧化酶影响的差异并探讨其意义。方法：用链脲佐菌素(STZ)复制糖尿病倾向小鼠模型(STZ鼠)，予以6周慢性多相性应激(限制、旋转、拥挤)，检测其空腹血糖(FBG)、空腹胰岛素(FINS)水平和胰、心、肝组织超氧化物歧化酶(SOD)、谷肽甘肽过氧化物酶(GSH-PX)活性及脂质过氧化物(LPO)含量。结果：慢性应激使STZ鼠FBG水平升高，FINS水平未增高；对STZ鼠各组织抗氧化酶活性有不同影响：加重其胰腺SOD活性下降和LPO含量升高，并使其GSH-PX活性降低(P均<0.05)，对其肝脏显示使SOD、GSH-PX活性升高和LPO含量下降的相反作用(P均<0.05)。结论：慢性应激及STZ诱导的糖尿病倾向协同作用特异地加重小鼠胰腺抗氧化酶活性下降和脂质过氧化物形成，可能参与损害其胰岛素分泌功能和导致高血糖症。
关键词：慢性应激 糖尿病 胰岛素 抗氧化酶 脂质过氧化物
分类号：R395.1 文献标识码：A
文章编号：1005-3611(2000)01-0019-04
Difference in Alterations of Antionxidant Enzyme Activities in Different Tissues of Diabetes-prone Mice by Chronic Exposure to Stressors and Its Implications
LIU Youshuo HU Min YAO Shuqiao et al,
（Hunan Medical University, Changsha）
Abstract：Objective：To investigate the degree of difference and direction of alterations of antioxidant enzyme activities in different tissues of normal and diabetes-prone animals by chronic stress and to explore its implications. Methods： The levels of fasting blood glucose (FBG) and fast plasma insulin (FINS), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and level of lipid peroxidate (LPO) were measured in the pancreas, heart and liver of normal and streptozotocin-induced diabetes-prone mice (STZ mice) exposed to multiple stressors (restrain, rotation, crowding) for 6 weeks. Results： The FBG level in STZ mice was elevated by stress, while the FINS level was not increased. In the pancreas of STZ mice, SOD activity was decreased and LPO formation increased (P<0.05); these negative changes resulting from STZ were exacerbated by chronic stress (P<0.05). However, opposite changes occurred in the liver of STZ mice after chronic stress (increased in SOD and GSH-PX activities, decreased in LPO content, P<0.05). On the other hand, chronic stress resulted in less change in normal mice (increased in SOD activity in heart, P<[1] [2] 下一页
上一个医学论文： 癌症患者生活质量状况及影响因素研究
下一个医学论文： 津医精神运动成套测验 JPB 的研制
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文