卵巢癌血清 腹水中白细胞介素

高　杰　文　杰　李守柔

　　中国图书分类号　R737.31　R730.3
　　摘　要　目的：探讨IL-6在卵巢上皮癌中的临床价值。方法：通过对40例卵巢癌患者血清、腹水中白细胞介素-6(Interleukin　6，IL-6)进行生物活性测定。结果：1、腹水中IL-6水平明显高于血清中IL-6水平(P＜0.0001)；2、腹水或血清中IL-6水平与卵巢癌分期呈正相关；3、卵巢癌术后残留与IL-6水平呈正相关；4、腹水中IL-6水平影响患者对卡铂化疗敏感性。结论：腹水或血清中IL-6生物活性的测定有可能成为卵巢癌疾病状态的评估及选择化疗药物的根据。
　　关键词　卵巢癌　MTT比色法　白细胞介素-6　血清　腹水

Clinical evaluation of interleukin-6 assay in serum and ascites of ovarian carainoma patients

GAO Jie，WEN Jie①，LI Shou-Rou①.
Department of Obstetrics and Gynecology，Peking Union Medical College Hospital，Chinese Acadeny of Medical Science，Beijing 100730．①Department of Obstetrics and Gynecology，the Second Clinical Hospiatl，Betheune Medical University，Changchun 130041

　　Abstract Objective：To study the clinical evaluation of IL-6 in ovarian epithelial carcinoma．Methods：With 7TD1 cell lines，MTT colovimetric method was applied to assay IL-6 activity in serum and ascites of ovarian carcinoma patients．Results：1．IL-6 activity in ascites is significantly greater than that in serum；2．IL-6 activity in ascites or serum positively correlated with stages of ovarian carcinoma；3．Residue after operation has significant correlation with IL-6 activity；4．IL-6 activity in ascites affected response of patients to chemotherapy．Conclusion：The assay of IL-6 activity in ascites or serum is expected to offer help for evaluating patients and selecting chemical drugs in ovarian epithelial carcinoma．
　　Key words Ovarian epithelian carcinoma MTT colorimetric method Interleukin-6 Serum Ascites

　　IL-6是一种多功能细胞因子，具有肿瘤生长刺激因子的作用，参与肿瘤转移及促进其恶化，血中IL-6水平升高提示多发性骨髓癌及肾细胞癌患者的预后较差［1，2］，但与卵巢癌关系目前尚不十分清楚。本文拟通过对卵巢癌患者腹水和血清中IL-6水平的测定，探讨IL-6在卵巢癌临床诊治中的价值。

1　材料与方法
1．1　资料来源　标本均来自1996年至1997年期间我院住院手术卵巢癌患者，组织病理学检查诊断浆液性囊腺癌24例，粘液性囊腺癌13例，未分化癌3例。
1.2　实验方法
1.2.1　标本取样　术前无菌抽取静脉血5 ml，不需抗凝，离心2 000 r/min 10 min，取上清液于Eppendorf管中密闭置于-40℃保存。术中取腹水5　ml，离心800 r/min 10 min，取上清液1 ml于Eppendorf管内密闭置于-40℃保存。检测时血清、腹水样品56℃灭活30 min后倍比稀释，血清样品最大稀释

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