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边缘系创伤反应性髓鞘碱性蛋白表达与超微结构观察 |
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王庆松 嘉 莉 欧阳尧 陈曼娥
摘 要 目的：探讨创伤反应性中枢神经系统(CNS)间接受损的可能机制。方法：借助犬双后肢低、高速投射物致伤模型，观察了边缘系下丘脑、海马及颞顶区脑组织髓鞘碱性蛋白(MBP)表达及超微结构改变。结果：伤后8 h低速组下丘脑MBP－mRNA表达增强(P＜0.01)；高速组下丘脑、海马MBP含量明显增高(P＜0.01)，MBP－mRNA表达增强(P＜0.01)；超微结构改变以高速组下丘脑、海马为著，多集中于神经元、神经纤维及毛细血管内皮细胞变化。结论：边缘系下丘脑、海马选择性易损，可能为创伤反应性CNS异常的重要病理生理基础。
主题词 大脑边缘系统；创伤和损伤；致脑炎碱性蛋白质类；超微结构
The expression of trauma reactive myelin basic protein and
observation of ultrastructure in limbic system
WANG Qing-Song,JIA Li,OU-Yang Yao，CHEN Man-E
Department of Neurology,Chengdu General Hospital,Chengdu(610083)
Abstract AIM:To explore the probable mechanism of trauma reactive effects on the central nervous system(CNS).METHODS:On the basis of stress model with different velocity steel bullet injury to hind thighs of dogs,the expression of myelin basic protein(MBP) in limbic system,temporoparietal gray and white matters,and the early changes of their ultrastructure were observed.RESULTS：The expression of MBP-mRNA in hypothalamus in low speed group was significantly enhanced(P＜0.05),MBP levels markedly increased(P＜0.01) and the expression of MBP-mRNA was enhanced remarkably (P＜0.01) in hypothalamus and hippocampus in high speed group 8 hours after trauma; and the early pathomorphism changes displayed some alterations in neurons, nerve fibers and capillary endothelium cells of limbic system in high grouop. CONCLUSION:Hypothalamus and hippocampus were vulnerably damaged in spite of no direct injury to head,and these may play a consistent pathophysiological role in the development of trauma reactive central nervous system disturbance.
MeSH Limbic system;Wounds and injuries;Encephalitogenic basic proteins; Ultrastructure
不少患者创伤虽未直接伤及大脑，却出现了一定程度的反应性神经、精神异常。部分伤员至创伤后期出现包括中枢神经系统(central nervous system,CNS)在内的多脏器功能衰竭［1，2］。认为可能与CNS间接受损有关［3，4］。鉴于目前探讨创伤反应性CNS异常的病理生理研究甚少，本实验借助犬双后肢低、高速投射物致伤模型，对边缘系下丘脑、海马及颞顶区灰、白质髓鞘碱性蛋白(myelin basic protein,MBP)的表达与释放进行定量分析，结合其早期超微[1] [2] [3] 下一页
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