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MSH27抗原在小鼠睾丸中的定位 |
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庄大中 宋祥芬 齐跃敏 孙青原 陈大元
【摘要】 目的 MSH27是与精、卵质膜融合相关的针对精子抗原的单克隆抗体，本研究检测了此抗体所识别的睾丸抗原分子及其分布。 方法 用免疫亲和层析和聚丙烯酰胺凝胶电泳纯化、分析了MSH27识别的小鼠睾丸抗原，并用免疫组织化学和免疫电镜进行了定位。 结果 单抗MSH27可识别睾丸提取蛋白中分子量为126kD和116kD的抗原分子，此抗原在睾丸的精原细胞至球形精子细胞的胞质内部均有表达，分布于内质网囊泡内或囊泡膜上。在精子形成过程中，此抗原由胞质内均匀分布变为聚集于顶体帽旁侧的区域，这一区域将形成顶体后区。 结论 126kD和116kD蛋白是单抗MSH27在睾丸中识别的抗原分子，它们可能是单抗MSH27在成熟精子中识别的39kD抗原的前体分子。
【关键词】 MSH27抗原 精子形成 定位
LOCATION OF THE MOUSE MSH27 ANTIGEN IN TESTES
Zhuang Dazhong Song Xiangfen Qi Yuemin Sun Qingyuan Chen DayuanΔ
(State Key Laboratory of Reproductive Biology, Institute of Zoology, Academia Sinica, Beijing)
【Abstract】 Objective MSH27 antigen plays an important role in the process of sperm-egg membrane fusion. In this study, the molecular weights and distribution of the antigen were investigated. Methods Immuno-affinity chromatography, SDS-polyacrylamide gel electrophoresis, immunohistochemistry and immunoelectron microscopy were used. Results Two proteins with the molecular weight of 126kD and 116kD were separated from the testicular extraction by immuno-affinity chromatography. It was demonstrated that the antigens were located in the endoplasmic reticulum, or in the vesicles which were released from the endoplasmic reticulum of all the spermatogenic cells, from spermatogonia to spermitids. In the process of the spermiogenesis, the antigens were assembled in the cytoplasm other than the acrosomal cap region, which was destined to form the postacrosomal region of the spermatozoon. Conclusion The 126kD and 116kD proteins, testicular antigens of the mAb MSH27 were deduced to be the precursors of the sperm antigens of mAb MSH27(M.W.39kD).
【Key words】 MSH27 antigen; Spermiogenesis; Location
抗小鼠精子蛋白的单克隆抗体MSH27在成熟精子中能识别分子量为39kD的抗原分子。过去的研究中发现，此抗原分子在精子顶体反应后位于精子与卵子质膜融合的起始区域即精子的赤道段和顶体后区；纯化的抗原分子及其单克隆抗体在体外受精实验中均可以阻止精、卵膜融合(待发表论文)。当精子在附睾成熟、获能和顶体反应过程中，精子MSH27抗原表位的逐渐暴露并与精子参与精、卵膜融合能力的增加同步(另一待发表论文)。因此，MSH27抗原可能在精、卵[1] [2] 下一页
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