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大鼠神经垂体催产素能神经元内神经分泌颗粒的免疫电镜观察 |
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刘胜洪 刘汉武 张敏海 刘能保 彭庆廉
【摘要】 目的 观察大鼠神经垂体催产素(OT)能神经元轴突及终末内颗粒囊泡形态学及免疫细胞化学特点。方法 应用电镜包埋后免疫细胞化学方法,对大鼠神经垂体进行OT免疫染色。结果 在OT能神经元轴突终末内,可见OT免疫反应性的致密核芯大颗粒囊泡和无OT免疫反应性的清亮小泡存在。部分囊泡与轴膜靠近,并可见颗粒囊泡与轴膜融合形成的胞吐现象;在轴突非终末部分,也可见OT免疫反应性的颗粒囊泡,散在分布轴浆内,还可见无OT免疫反应性的颗粒囊泡,呈散在或聚集分布,两种囊泡均可位于微管之间。结论 位于轴突和轴突终末内的囊泡,在形态学和OT免疫染色方面有明显差异,推测它们在功能上也有不同。 【关键词】 神经垂体 催产素 神经分泌颗粒 免疫电镜
IMMUNOELECTRON MICROSCOPIC OBSERVATION FOR NEUROSECRETORY GRANULES OF OXYTOCINERGIC FIBER IN NEUROLOBE OF RAT HYPOPHYSIS
Liu ShenghongΔ,Liu Hanwu,Zhang Minhai,Liu Nengbao,Peng Qinglian (Department of Histology and Embryology,Tongji Medical University,Wuhan)
【Abstract】 Objective To observe the morphological and immunocytochemical characteristic of the secretory granules within the axon and axonal terminal of oxytocinergic neurons.Methods Postembedding immunocytochemistry for electron microscopy was used for labelling oxytocinergic neurofiber in neurolobe of rat hypophysis.Results The large dense core granules with oxytocin(OT) immunoreactivity and clear microvisicles without OT immunoreactivity were observed in the axonal terminal of the neuron.Some OT positive granules were fused with the membrane of the terminal and exocytosis of the granules were demonstrated.In the rest part of axon,the scattered or packaged granules without OT immunoreactivity and scattered granules with OT immunoreactivity were found to be located at the space between microtubes of the axon.Conclusions Because there are some morphological and immunocytochemical difference between the granules in axon and axonal terminal,there might be some functional difference between them. 【Key words】 Neurolobe of hypophysis;Oxytocin;Neurosecretory granule;Immunoelectron microscopy
囊泡是分泌性神经元内胞体和轴突之间进行物质运输的重要结构成分,与之有关的研究一直受到学者的重视[1~4]。尽管有人曾报道,输送至神经垂体不同部位神经纤维内的囊泡对渗透压具敏感性,以及囊泡内物质的理化性质等方面存在着明显差异[2,5],但对轴突不同部位中囊泡的超微结构、分布以及免疫反应性等方面是否也存在着相应差异,文献报道较少。近年来,随着免疫电镜技术方法的不断改进[1] [2] 下一页 上一个医学论文: 大鼠脑垂体前叶内IL 下一个医学论文: 白细胞介素6受体样免疫反应性细胞在大鼠脑内的分布
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