|
局灶性脑缺血后鼠脑组织tPA基因表达及其与细胞凋亡 |
| |
潘涌 郑素秋 谢勉 谢元云 林以理 颜光美
摘 要：目的 探讨局灶性脑缺血后组织型纤溶酶原激活物(tPA)基因的表达及其与细胞凋亡的关系。 方法 用原位杂交和免疫组织化学方法检测tPA基因在大鼠局灶性脑缺血(大脑中动脉阻塞)后的表达，用TUNEL法检测缺血不同时间的细胞凋亡状况。 结果 脑缺血6h缺血中心部位的胶质细胞和梗死灶周边缺血半影区的神经元均观察到tPA免疫反应性信号，在大脑皮层后肢体区、顶皮层区、海马区和齿状回可见凋亡细胞，但缺血18h缺血侧神经元及胶质细胞未见tPA免疫反应性信号，仅齿状回有凋亡细胞。 结论 tPA蛋白在脑缺血6h缺血侧表达增高，局灶性脑缺血后神经元死亡有细胞凋亡的参与，该凋亡可能与tPA基因表达和缺血时间有一定关联。
关键词：组织型纤溶酶原激活物；脑缺血；表达；基因
分类号：Q344+.13； Q255 文献标识码：A
文章编号：0529-1356(2000)01-13
EXPRESSION OF tPA GENE AND CELL APOPTOSIS FOLLOWING
FOCAL CEREBRAL ISCHEMIA IN RAT
PAN Yong
(Department of Pharmacology)
ZHENG Su-qiu
(Department of Pharmacology)
XIE Mian
(Department of Anatomy,Sun Yat-sen University of Medical Sciences,Guangzhou 510089;China)
XIE Yuan-yun
(Department of Anatomy,Sun Yat-sen University of Medical Sciences,Guangzhou 510089;China)
LIN Yi-li
(Department of Anatomy,Sun Yat-sen University of Medical Sciences,Guangzhou 510089;China)
YAN Guang-mei
(Department of Pharmacology)
Abstract：Objective To study the expression of tissue plasminogen activator(tPA) gene and the relationship with cell apoptosis following focal cerebral ischemia in rat.Methods The samples of focal cerebral ischemia(middle cerebral artery occlusion,MCA occlusion) were analyzed by using in situ hybridization (ISH) and immunohistochemical(IHC) methods.Apoptotic neurons were detected by using TdT-mediated dUTP nick end labeling (TUNEL).Results In the rats 6h after MCA occlusion,posititive immunostaining of tPA was detected in endothelial cells of small blood vessels,meninges,chorioid,in the glial cells at the zone of ischemic core and neurons at the infarct border zone,apoptotic cells were observed in parietal and hindlimb cerebral cortex,hippocumpus and dentate gyrus,but in the rats 18h after MCA occlusion,immunoreactivity of tPA was not detected in the neurons and glial cells of the ischemic hemisphere,and apoptotic cel[1] [2] 下一页
上一个医学论文： 成年大鼠纹状体区神经干细胞的分离培养
下一个医学论文： 突触数密度Disector测量法的建立与计算机辅助分析的实现
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文