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线粒体DNA与人精子活力间的相关性分析 |
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郑英 周作民 沙家豪
摘 要：目的 探讨线粒体DNA与人精子活力间的关系。 方法 用长链PCR技术，对60例精子活力正常和40例精子活力异常不育患者的精子线粒体DNA(mtDNA)进行了多重缺失的分析。 结果 两组不育患者中共有8例具有mtDNA的多重缺失(其中精子活力正常不育患者6名，精子活力异常不育患者2名)，但缺失型mtDNA(S5除外)在总mtDNA中所占比例很小(0.16%～1.85%)，1例精子活力正常的不育患者(S5)具有2.6kb的缺失型mtDNA，且缺失型mtDNA占总mtDNA的91.02%，其序列分析发现mtDNA编码区域大部分缺失。 结论 精子活力与mtDNA的多重缺失间无相关性。
关键词：线粒体DNA；精子活力；长链PCR
分类号：Q523 文献标识码：A
文章编号：0529-1356(2000)01-34
ANALYSIS OF THE CORRELATION BETWEEN MITOCHONDRIAL
DNA AND HUMAN SPERM MOBILITY
ZHENG Ying
(Department of Histolog and Embryology,Medical College of Yangzhou University,Yangzhou 225001,China)
ZHOU Zuo-min
(Key Laboratory of Jiangsu Reproductive Medicine,Nanjing Medical University,Nanjing 210029,China)
SHA Jia-hao
(Key Laboratory of Jiangsu Reproductive Medicine,Nanjing Medical University,Nanjing 210029,China)
Abstract：Objective To analyze the correlation between mitochondrial DNA and human sperm mobility. Methods By using long polymerase chain reaction techniques,we studied multiple deletions of sperm mitochondrial DNA(mtDNA) in 100 infertile patients(60 with normal sperm mobility and 40 asthenospermia). Results Six of 60 patients with normal sperm mobility and two of 40 asthenospermia had multiple mtDNA deletions,but percentage of deletions in mtDNA(DmtDNA) in total mtDNA was very low(from 0.16% to 1.85%).A 2.6kb DmtDNA was found in one of infertile patients (No.S5) with normal sperm mobility,and percentage of 2.6kb DmtDNA in total mtDNA was 91.02%.Sequence analysis revealed that most of mtDNA sequence encoding RNA and polypeptide were deleted. Conclusion There is no relationship between multiple deletions in mtDNA and human sperm mobility.
Key words：Mitochondrial DNA; Sperm mobility; Long polymerase chain reaction▲
线粒体是真核生物细胞内能量转换体系和供能中心，它含有一个独立的半自主复制的DNA，称为线粒体DNA(mitochondrial DNA,mtDNA)。mtDNA是16.569kb的闭环双链DNA分子，编码氧化磷酸化(oxidative phosphorylation,OXPHOS)中的13种多肽，这[1] [2] 下一页
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