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急 慢性缺氧对大鼠脑线粒体能量代谢的影响 |
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高文祥 柳君泽 吴利平 蔡明春
［摘 要］ 目的：探讨缺氧大鼠脑线粒体能量代谢的特点。方法：雄性Wistar大鼠随机分为急性缺氧组（AH）、慢性缺氧组（CH）和对照组。急、慢性缺氧组动物分别连续暴露于模拟4 000 m高原3 d（AH）和40 d（CH）。分离脑线粒体，分别测定线粒体呼吸功能、线粒体内腺苷酸池含量、ATP生成能力和F0F1-ATP酶活性。结果：急性缺氧大鼠IV态呼吸（ST4）显著升高，伴呼吸控制率（RCR）降低，同时线粒体内ATP含量、ATP生成率和F0F1-ATP酶活性均显著降低；慢性缺氧大鼠ST4、RCR、线粒体ATP含量和F0F1-ATP酶活性部分恢复。结论：急性缺氧脑线粒体代谢是以功能受损为特点，而慢性缺氧时则表现为功能的部分代偿。
［主题词］ 低氧； 线粒体； 氧化磷酸化； 能量代谢； 脑
［中图分类号］ R364.4 ［文献标识码］ A
［文章编号］ 1000-4718(2000)10-0879-04
Characteristics of energy metabolism in brain mitochondria
of rats exposed to hypoxia
GAO Wen-xiang
（Department of Pathophysiology & Institute of High Altitude, the Third Military Medical University,Chongqing 400038，China）
LIU Jun-ze
（Department of Pathophysiology & Institute of High Altitude, the Third Military Medical University,Chongqing 400038，China）
WU Li-ping
（Xijing Hospital, the 4th Military Medical University, Xian 710032, China） CAI Ming-chun
（Department of Pathophysiology & Institute of High Altitude, the Third Military Medical University,Chongqing 400038，China）
［Abstract］ AIM:To explore the characteristics of energy metabolism in brain mitochondria of rats exposed to acute and chronic hypoxia. METHODS: Animal grouping: Wistar rats were randomized into acute hypoxic group (AH), chronic hypoxic group (CH) and the control. Respiratory function, F0F1-ATPase activity, mitochondrial ATP, ADP and AMP contents and ATP production rate were measured respectively. RESULTS: In AH, brain mitochondrial respiratory state IV (ST4) was increased, while respiratory control rate (RCR), mitochondrial ATP content, ATP production rate and F0F1-ATPase activity were decreased respectively. In CH, ST4, RCR, mitochondrial ATP content and F0F1-ATPase activity were reversed partially. CONCLUSION: Acute hypoxia may impair brain mitochondria energy metabolism by way of depressing mitochondrial oxidative phosphorylation and AT[1] [2] 下一页
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