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血管内皮生长因子及其基因在自发性高血压大鼠心肌中的表达 |
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李夏 李昭波 高云秋 杨萍 李维根 唐朝枢
摘 要 目的：血管内皮生长因子(VEGF)是新近确定的一 种特异作用于血管内皮细胞的活性肽。最近发现正常心肌细胞有VEGF及其基因表达，但对高 血压肥大心脏心肌VEGF及其基因表达的变化尚不清楚。方法：本实验采用 免疫组化和分子杂交方法，对自发性高血压大鼠(SHR)肥大心脏心肌VEGF及其基因表达进行 研究。结果：免疫组化结果表明，SHR心肌细胞浆内特异性VEGF染色颗粒明 显多于WKY对照大鼠。Northern分子杂交结果表明，SHR心肌VEGF mRNA表达明显强于WKY对照 大 鼠。结论：目前对这一结果的生理意义还不清楚，推测可能与心肌肥大时 细胞间质血管增生有关。
主题词 生长物质；内皮，血管；心肌；肥大；高血压
Expression of vascular endothelial growth factor and its
gene in myocardium of spontaneously hypertensive rats
LI Xia, LI Zhao-Bo, GAO Yun-Qiu, YANG Ping, LI Wei-Gen, TANG Zhao S hu*
Institute of Sport Medicine, Institute of Cardiovascular Research*,
Bei jing Medical University, Beijing (100083)
Abstract AIM：Vascular endothelial growth factor(VEGF), a growt h and permeability factor specific for endothelial cell, has been identified in m yocardium of the normal adult heart, but the change of VEGF in myocardium of hyp ertensive hypertrophic heart was unclear. METHODS：We therefor stu d ied the expression of VEGF and its gene in myocardium of spontaneously hypertens ive rats(SHR) by immunohistochemistry assay and Northen bolt. The results were analyzed by scanning densitometry.RESULTS：Compared with control W istar Kyoto (WKY) rats, the left ventricle weight/body weight of SHR increased b y 61%(P＜0.01). The intracellular VEGF immunoactivity was present in myocard ium of WKY rats and SHR, but the immunoactivity of SHR was 91% strong er than that of WKY rats. The results of Northern bolt showed that the expressio n of VEGF mRNA of SHR increased by 67% than that of WKY by scanning densitometr y. CONCLUSION：Although little is known about the role of expressi on upregulation of VEGF and its gene in myocardium of SHR, it seems to respond to vascular proliferation of hypertrophic heart.
MeSH Growth substances; Endothelium, vascular; Myocardium; Hyper trophy; Hypertension
心肌肥大是心脏自身针对血流动力学因素改变，通过自分泌和旁分泌 调节而发生的一种适应性变化［1］。大量研究表明血管紧张素[1] [2] 下一页
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